We narrowed to 3,949 results for: SPL

1. SFG.wtCNa_opt.IRES.eGFP

   Plasmid

   #22489

   Depositor

   Martin Pule

   Article

   Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

   Insert

   codon optimised wild type Calcineurin A (PPP3CA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon Optimised wtCNa

   Promoter

   Available since

   Dec. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-GFP11-pDEST-C

   Plasmid

   #118367

   Purpose

   Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   GFP11-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET26b_Nb.b201

   Plasmid

   #131404

   Purpose

   E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

   Depositor

   Andrew Kruse

   Article

   McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

   Insert

   Nanobody b201
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. DT480

   Plasmid

   #80413

   Purpose

   Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

   Depositor

   Thomas Cooper

   Article

   Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

   Insert

   human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. L1-neo-TET

   Plasmid

   #51284

   Purpose

   expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   L1RE1 (L1RE1 Human)
   

   Use

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. FlagETR3 (CELF2)

   Plasmid

   #96900

   Purpose

   expression of Flag-tagged human CELF2 (aka CUGBP2)

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   human CELF2 cDNA (CELF2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDAS12489_PEAR-GFP_2in1_2.0

   Plasmid

   #177186

   Purpose

   PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Intron-Tagging-EGFP-Donor

   Plasmid

   #159740

   Purpose

   Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   EGFP
   

   Use

   Intron tagging donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-hPMCA4b

   Plasmid

   #47589

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA4x/b (ATP2B4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K442R and P673L relative to NP_001675.3

   Promoter

   CMV

   Available since

   Aug. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGK61

    Plasmid

    #228121

    Purpose

    Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-GFP11-pDEST-N

    Plasmid

    #118366

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    GFP11-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p10xUAS-luc2

    Plasmid

    #173006

    Purpose

    firefly luciferase reporter vector with 10x clustered UAS binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    10xUAS-luc2
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. DDX5 WT V5

    Plasmid

    #199576

    Purpose

    Expresses WT DDX5 cloned into pLenti puro

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-pEF1a-EBFP-tTAn

    Plasmid

    #172122

    Purpose

    Constitutive expression of EBFP and rtTA-InteinN

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    EBFP, tTAn-InteinN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. (112) pcDNA3.1-GFP-Flag-Nup62-EPEA

    Plasmid

    #168101

    Purpose

    GFP and Flag tagged Nup62 with EPEA nanobody tag

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    GFP-Flag-Nup62-EPEA
    

    Use

    Tags

    EPEA, Flag, and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

    Plasmid

    #172067

    Purpose

    Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCTCON2 Aga2P-HRP

    Plasmid

    #73152

    Purpose

    Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Aga2P-HRP
    

    Use

    Tags

    HA tag and myc tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDualEX-SMN2

    Plasmid

    #214431

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. (294) pcDNA3.1-myc-OGA(1-400)

    Plasmid

    #168095

    Purpose

    Myc tagged truncated human OGA catalytic domain of residues 1-400

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    myc-OGA(1-400)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6_(GLuc)_INT[3xMS2_SL]

    Plasmid

    #68426

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three MS2 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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