We narrowed to 5,985 results for: actin

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lentiCRISPR v2-sgHSPC300-2

Plasmid

#210142

Purpose

knock out HSPC300 in mammalian cells

Depositor

Boyi Gan

Publication

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Protein BRICK1 (BRK1 Human)

Use

CRISPR

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR N52R (Shank3)

Plasmid

#175255

Purpose

SPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Publication

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with N52R mutation from SHANK3

Expression

Mammalian

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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human TLNRD1-4H pET151

Plasmid

#159386

Purpose

Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

Depositor

Ben Goult

Publication

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 (TLNRD1 Human)

Tags

his-tag, TEV cleavage site

Expression

Bacterial

Mutation

TLNRD1 4-helix domain (residues 143-273)

Promoter

T7

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHD-dsRed-attP_dCrk_flankingHRs

Plasmid

#131141

Purpose

Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

Depositor

Mark Peifer

Publication

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Inserts

Crk Upstream Homology Region (Crk Fly)
Crk Downstream Homology Region (Crk Fly)

Use

CRISPR and Cre/Lox; Homology directed repair temp…

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Publication

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA2

Plasmid

#131140

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Publication

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GCaMP6m.WPRE.SV40

Plasmid

#100840

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Publication

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has service

AAV1

Insert

GCaMP6m

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

Promoter

CAG

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-RIPK2

Plasmid

#23846

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIPK2 (RIPK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-HIPK2

Plasmid

#23481

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HIPK2 (HIPK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCMV4-HA

Plasmid

#27553

Depositor

Shao-Cong Sun

Publication

Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

Type

Empty backbone

Tags

3x HA

Expression

Mammalian

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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LifeAct-mGarnet

Plasmid

#104307

Purpose

codes for a fusion of fluorescent protein mGarnet and lifeact that targets F-actin in live cells

Depositor

Ulrich Nienhaus

Publication

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

LifeAct-mGarnet

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pclbw-opa1(isoform 1)-myc

Plasmid

#62845

Purpose

Mammalian Expression of myc-tagged Opa1 isoform1

Depositor

David Chan

Publication

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mmOPA1isoform1-myc (Opa1 Mouse)

Use

Retroviral

Tags

9xMyc

Expression

Mammalian

Promoter

cmv-bactin

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMRX-IPU-GFP-MYH9

Plasmid

#168273

Purpose

Expresses MYH9 in mammalian cells

Depositor

Noboru Mizushima

Publication

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

MYH9 (MYH9 Human)

Tags

FLAG

Expression

Mammalian

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-FilaminA-N-9

Plasmid

#57379

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

FilaminA (FLNA Human)

Tags

mEos2

Expression

Mammalian

Mutation

D2626H

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Dendra2-VASP-5

Plasmid

#57744

Purpose

Localization: Actin/Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

VASP

Tags

Dendra2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRK5-mFzd7

Plasmid

#42259

Depositor

Chris Garcia, Jeremy Nathans

Publication

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 7 (Fzd7 Mouse)

Expression

Mammalian

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pRK5-mWnt5a

Plasmid

#42279

Depositor

Chris Garcia, Jeremy Nathans

Publication

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt5a (Wnt5a Mouse)

Expression

Mammalian

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
GIT1A-FLAG

Plasmid

#15225

Depositor

Rick Horwitz

Publication

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

flag

Expression

Mammalian

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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