We narrowed to 7,294 results for: ef1a

1. iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

   Plasmid

   #204723

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLQ11438

   Plasmid

   #183204

   Purpose

   pHR-EF1a-ABE8e-hyperdLbCas12a-NLS-mCherry-P2A-Puro-WPRE

   Depositor

   Stanley Qi

   Article

   Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

   Insert

   hyperdCas12 base editor
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-dCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201152

   Purpose

   Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   dCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mutation

   gRNA sequence: TACGTTCTCTATCACTGAT

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSBbi-BB

   Plasmid

   #60521

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSBbi-GH

   Plasmid

   #60514

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and hygromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSBbi-BH

   Plasmid

   #60515

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and hygromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSBbi-BN

   Plasmid

   #60518

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-AP.Adamts2.1-GFP-WPRE

   Plasmid

   #211488

   Purpose

   expresses GFP under artificial promoter Adamts2

   Depositor

   Georg Keller

   Article

   O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKER-mKOk

   Plasmid

   #85975

   Purpose

   Expresses mKOk from the EF1a promoter in mammalian cells

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   mKOk
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAF124

    Plasmid

    #222372

    Purpose

    Recombinant human IgG1 antibody (3D5) that binds to 6xHis at the C-terminus of proteins

    Depositor

    Andrew S. Flies

    Article

    Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

    Insert

    IGHG
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKER-mRuby2

    Plasmid

    #85977

    Purpose

    Expresses mRuby2 from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKER-mCardinal

    Plasmid

    #85976

    Purpose

    Expresses mCardinal from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGH916

    Plasmid

    #230842

    Purpose

    Ef1a-DivA-BE3-T2A-tagBFP-wPRE

    Depositor

    Gaelen Hess

    Article

    Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    DivA-BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGH912

    Plasmid

    #230855

    Purpose

    Ef1a-DivA-BE3-T2A-BlastR-wPRE

    Depositor

    Gaelen Hess

    Article

    Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    DivA-BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB-mCherry-SopF

    Plasmid

    #229745

    Purpose

    Expresses mCherry-tagged SopF in mammalian cells. Stable expression with piggybac transposase.

    Depositor

    Shawn Ferguson

    Article

    Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

    Insert

    mCherry-SopF
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGH907

    Plasmid

    #230837

    Purpose

    Ef1a-MS2-AIDDead-T2A-GFP-wPRE

    Depositor

    Gaelen Hess

    Article

    Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    MS2-AIDDead
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGH518

    Plasmid

    #230836

    Purpose

    Ef1a-MS2-AID*-T2A-GFP-wPRE

    Depositor

    Gaelen Hess

    Article

    Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    MS2-AID*
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGH896

    Plasmid

    #230840

    Purpose

    Ef1a-DivA-BE-T2A-tagBFP-wPRE

    Depositor

    Gaelen Hess

    Article

    Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    DivA-BE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGH397

    Plasmid

    #230853

    Purpose

    Ef1a-AID*-n'Cas9-T2A-BlastR-wPRE

    Depositor

    Gaelen Hess

    Article

    Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    AID*-n'Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGH911

    Plasmid

    #230856

    Purpose

    Ef1a-BE3-T2A-BlastR-wPRE

    Depositor

    Gaelen Hess

    Article

    Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    BE3 (rAPOBEC1)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge