We narrowed to 5,842 results for: pCas

1. p2T-cmv-SpCas9-TadCBEa-BlastR

   Plasmid

   #193847

   Purpose

   Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEa
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-PDGFRb559-562del-IRES2-EGFP

   Plasmid

   #204355

   Purpose

   Expresses a mutant PDGFRb gene (559-562 deletion).

   Depositor

   Hirofumi Nakatomi

   Article

   Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

   Insert

   Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
   

   Mutation

   559-562 deletion

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-FLEX-fwd[Chrimson-tdT]

   Plasmid

   #59137

   Purpose

   Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chrimson-tdTomato
   

   Use

   Cre/Lox

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-EGFP/RFP-miR-Scrint

   Plasmid

   #19821

   Depositor

   Zuoshang Xu

   Article

   Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

   Insert

   Scrambled miRNA
   

   Use

   Cre/Lox and RNAi

   Expression

   Mammalian

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pspCas9-3'UTR-ST2-com-vector

   Plasmid

   #136269

   Purpose

   Expresses SpCas9 and sgRNA Scaffold

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Insert

   SpCas9 and sgRNA scaffold
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV pCAG-FLEX-mRuby3-WPRE

   Plasmid

   #99279

   Purpose

   Can be used to generate AAV virus that will express mRuby3 in the presence of Cre

   Depositor

   Rylan Larsen

   Insert

   mRuby3
   

   Use

   AAV and Cre/Lox

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

   Plasmid

   #133044

   Purpose

   Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

   Insert

   Xph20-ETWV
   

   Tags

   NLS, TEV protease + TEV cleavage site, and miRFP6…

   Expression

   Mammalian

   Mutation

   S63K

   Promoter

   CAG

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126698

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   sgRNA targeting human SCGB3A2 locus
   

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS SARS-CoV-2 Alpha Spike

   Plasmid

   #185691

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

   Depositor

   Marceline Côté

   Article

   Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

   Insert

   SARS-CoV-2 Alpha Spike (S )
   

   Expression

   Mammalian

   Mutation

   Contains the following mutations: Δ69-70, Δ145, N…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available Since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

    Plasmid

    #196829

    Purpose

    Express dpdpCas13b with AAAA mutation and Clover

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NRCH-APOBEC-nCas9-Ung

    Plasmid

    #198552

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-TWIN-STREP-FLAG-FLCN

    Plasmid

    #164981

    Purpose

    FLCN expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FLCN (FLCN Human)
    

    Tags

    2xSTREP FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-mCherry-FKBP-GAP(ARHGAP29)

    Plasmid

    #222632

    Purpose

    Rho inactivation using rapamycin

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
    

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

    Plasmid

    #176458

    Purpose

    Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Expression

    Mammalian

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-FLAG-IRES-blasticidin-Sox2 WT

    Plasmid

    #182046

    Purpose

    expression of Sox2

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-OSF-PP-human-CHMP3(150)

    Plasmid

    #154181

    Purpose

    expresses human CHMP3(150) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (CHMP3 Human)
    

    Tags

    FLAG and Strep-Tag II

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 150

    Promoter

    CAG

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-miRFP670-pA_U6-BbsI (pE059)

    Plasmid

    #149353

    Purpose

    Mammalian expression vector for miRFP670

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    miRFP670
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-Twin-Strep-TEV-DGKζ

    Plasmid

    #202531

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    DGKZ (DGKZ Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-H2B-mRuby3-IRES-eGtACR1-ST

    Plasmid

    #108960

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eGtACR1-ST
    

    Expression

    Mammalian

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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