Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,672 results for: pCas

Clear keywords
Showing: 2161 - 2180 of 2672 results

  1. pEvolvR-mCherry-enCas9(H840A)-PolI5M

    Plasmid
    #249064
    Purpose
    Expresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enCas9(H840A)-PolI5M
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    249064-plasmid-map-sequence-id-495619

    Enlarge

  6. sgALK(Chr. Inv.)

    Plasmid
    #85569
    Purpose
    Expresses sgRNA targeting human ALK between exon 20 and 21
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    anaplastic lymphoma receptor tyrosine kinase (ALK Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  8. pNOC_dCas9_sgRNA Td2

    Plasmid
    #176258
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176258-plasmid-map-sequence-id-347041

    Enlarge

  9. Tad-CBE2.4

    Plasmid
    #235055
    Purpose
    Expresses Tad-CBE2.4 in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE2.4-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235055-plasmid-map-sequence-id-466794

    Enlarge

  10. Tad-CBE3.6

    Plasmid
    #235058
    Purpose
    Expresses Tad-CBE3.6 in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE3.6-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235058-plasmid-map-sequence-id-466807

    Enlarge

  11. ABE-RA2.1

    Plasmid
    #208300
    Purpose
    Expresses ABE-RA2.1 in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-RA2.1-SpCas9 D10A
    Expression
    Mammalian
    Mutation
    P48A, R51H, D108G, K110R, H122R, M126I, N127K
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    208300-plasmid-map-sequence-id-410378

    Enlarge

  13. PX459v2-ILK-gRNA 1-exon 1

    Plasmid
    #163320
    Purpose
    SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.
    Depositor
    Val Brunton
    Publication
    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ILK gRNA 1_Exon1
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only

  14. JPF0454

    Plasmid
    #124039
    Purpose
    Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pHR_pPGK1-spCAS9-P2A-mAzamiGreen
    Expression
    Mammalian
    Available since
    Feb. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    124039-plasmid-map-sequence-id-272811

    Enlarge

  16. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid
    #199254
    Purpose
    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity S. pyogenes Cas9-D10A-KARA nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A R1060A
    Promoter
    CAG promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199254-plasmid-map-sequence-id-391656

    Enlarge

  17. pGG438

    Plasmid
    #165486
    Purpose
    Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, S1136R, D1332K, and R1333G mutations in S…
    Promoter
    CMV and T7
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165486-plasmid-map-sequence-id-330963

    Enlarge

  18. Tad-CBE(CCA3)

    Plasmid
    #235070
    Purpose
    Expresses Tad-CBE(CCA3) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(CCA3)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235070-plasmid-map-sequence-id-466792

    Enlarge

  19. Tad-CBE(CCC2)

    Plasmid
    #235072
    Purpose
    Expresses Tad-CBE(CCC2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(CCC2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235072-plasmid-map-sequence-id-469791

    Enlarge

  20. Tad-CBE(GCC3)

    Plasmid
    #235078
    Purpose
    Expresses Tad-CBE(GCC3) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(GCC3)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235078-plasmid-map-sequence-id-469792

    Enlarge

Showing: 2161 - 2180 of 2672 results