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Plasmid#170406PurposeGenetic complementation plasmid of LetA-[LetB PLL6->1] mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ702-717, replace with 114-130)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only
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pBEL1284
Plasmid#175960PurposeFor expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
UseTags6xHis 2xQH and TEV cleavage site on LetAExpressionBacterialMutationPromoteraraBADAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2114
Plasmid#175961PurposeFor expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
UseTags8xHis 2xQH and TEV cleavage site on LetAExpressionBacterialMutationΔ634-746PromoteraraBADAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCX-EGFP beta5 integrin receptor
Plasmid#14996DepositorInsertbeta5 integrin receptor (ITGB5 Human)
UseTagsIRES EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKB33 (pUC19-KANMX-tetO7-CYCpr)
Plasmid#210472PurposePlasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.DepositorInsertKanMX-TetO7-CYCpr cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HeLa full-length MLCK-GFP
Plasmid#46316DepositorInsertHeLa long myosin light chain kinase (MYLK Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB11 (pDEST-DHFR F[1,2] C-term, NatR)
Plasmid#210485PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
Plasmid#210487PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB12 (pDEST-DHFR F[3] C-term, HygR)
Plasmid#210486PurposepDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0617 (pDEST-mEGFP C-term, NatR)
Plasmid#210484PurposepDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsmEGFPExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2265
Plasmid#175962PurposeFor expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
UseTags8xHis 2xQH and TEV cleavage site on LetAExpressionBacterialMutationΔ347-361, replace with 702-716PromoteraraBADAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH3(2)-4R
Plasmid#112090PurposeBacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-4R (NCK1 Human)
UseTagsGSTExpressionBacterialMutationPromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH3(2)-3R
Plasmid#112091PurposeBacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-3R (NCK1 Human)
UseTagsGSTExpressionBacterialMutationPromoterLacAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hela kinase-dead MLCK-GFP
Plasmid#46317DepositorInsertHeLa long myosin light chain kinase-dead (MYLK Human)
UseTagsGFPExpressionBacterial and MammalianMutationE1626KPromoterCMVAvailable sinceJuly 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-SH3(5R)
Plasmid#112154PurposeMammalian expression plasmid for SH3-5R in a vector expressing mCherry.DepositorInsertSH3-5R (NCK1 Human)
UseTagsExpressionMammalianMutationonly the second SH3 domain is expressed in this c…PromoterCMVAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DEPTOR-WT
Plasmid#159159PurposeExpresses DEPTOR in mammalian cellsDepositorInsertDEPTOR (DEPTOR Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK354
Plasmid#129698PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120010799
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-NT4
Plasmid#123328PurposeLentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertnon-targeting control shRNA embedded in miR30 backbone
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only