We narrowed to 4,541 results for: Alk

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pNK116

Plasmid

#219742

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal hispidin-3-hydroxylase

Use

Synthetic Biology

Mutation

D37E, V181I, S323M, M385K

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

Plasmid

#218914

Purpose

PiggyBac transposition

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )

Expression

Mammalian

Mutation

2363G>C and 4330T>C

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-NEDD1-gTBD

Plasmid

#196858

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-mCRT-GPI-IRES-DsRed

Plasmid

#223711

Purpose

Mammalian expression of mouse CRT-GPI

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin-GPI (Calr Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBactin-EGFP-NEDD1-gTBD

Plasmid

#196857

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

EGFP-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc FL

Plasmid

#221853

Purpose

Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

calreticulin (CALR Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID

Plasmid

#122982

Purpose

AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT

Depositor

Matthew Kennedy

Article

Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

Insert

mCh-IRES-BoNT/B(1-146)-iLID

Use

AAV

Tags

mCh

Expression

Mammalian

Mutation

iLID contains long lived V416I mutation

Promoter

human synapsin

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-DENV_NS3-GS-NS2B

Plasmid

#201940

Purpose

Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

DENV NS3-GS-NS2B (POLY )

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-WNV_NS2B-GS-NS3

Plasmid

#203475

Purpose

Expresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

WNV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SARS-CoV-2_PLP

Plasmid

#203509

Purpose

Expresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV-2 PLP (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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BE3-NLS-NLS-P2A-BLAST

Plasmid

#135348

Purpose

Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

BE3-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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EphA2 430-976 pcDNA3.1

Plasmid

#102738

Purpose

Expression of c terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (430-976aa) (Epha2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pET51b-Strep-hAGT-His

Plasmid

#167276

Purpose

Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

hAGT (AGT Human)

Tags

His x10 and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_LacZ

Plasmid

#155103

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIKneo 3XFLAG-wtGATA6-3XAU1_RNAi resistant

Plasmid

#72616

Purpose

Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Lentiviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Promoter

TRE tight

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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LCV2_BFP2

Plasmid

#155097

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsGIGYF2_AE

Plasmid

#148550

Purpose

Mammalian Expression of HsGIGYF2

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2 (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_AE

Plasmid

#148577

Purpose

Mammalian Expression of HsGIGYF2

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2 (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_LacZ_sgRNA

Plasmid

#155108

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant

Plasmid

#72922

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Entry vector for gateway cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Promoter

TRE tight

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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