Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,672 results for: pCas

Clear keywords
Showing: 2181 - 2200 of 2672 results

  1. Tad-CBE(GCG4)

    Plasmid
    #235080
    Purpose
    Expresses Tad-CBE(GCG4) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(GCG4)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235080-plasmid-map-sequence-id-466787

    Enlarge

  2. Tad-CBE(ACA1)

    Plasmid
    #235059
    Purpose
    Expresses Tad-CBE(ACA1) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACA1)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235059-plasmid-map-sequence-id-466805

    Enlarge

  3. Tad-CBE(ACC3)

    Plasmid
    #235062
    Purpose
    Expresses Tad-CBE(ACC3) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACC3)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235062-plasmid-map-sequence-id-466793

    Enlarge

  4. pYPQAT01

    Plasmid
    #223373
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223373-plasmid-map-sequence-id-454171

    Enlarge

  5. pYPQAT02

    Plasmid
    #223374
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223374-plasmid-map-sequence-id-452305

    Enlarge

  6. TadA-NG-CBE

    Plasmid
    #227420
    Purpose
    C-to-T base editing in mammalian cells
    Depositor
    Yang Sun
    Publication
    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-nSpCas9-NG-2xUGI (AICDA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227420-plasmid-map-sequence-id-450076

    Enlarge

  7. psgRNA-GST-EGFP-GPIDAF

    Plasmid
    #213719
    Purpose
    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.
    Depositor
    Qilai Huang
    Publication
    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Use
    CRISPR
    Tags
    GST
    Promoter
    CMV
    Available since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213719-plasmid-map-sequence-id-429329

    Enlarge

  8. ABE-RA2.0

    Plasmid
    #208299
    Purpose
    Expresses ABE-RA2.0 in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-RA2.0-SpCas9 D10A
    Expression
    Mammalian
    Mutation
    P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    208299-plasmid-map-sequence-id-410373

    Enlarge

  9. ABE-RA3.1

    Plasmid
    #208302
    Purpose
    Expresses ABE-RA3.1 in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-RA3.1-SpCas9 D10A
    Expression
    Mammalian
    Mutation
    E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    208302-plasmid-map-sequence-id-410372

    Enlarge

  10. HiFi iFE

    Plasmid
    #190141
    Purpose
    Expresses HiFiCas9-POLB (HiFi iFE)
    Depositor
    Timothy Lu
    Publication
    Nakade et al bioRxiv 2022.12.05.518807
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi Cas9-POLB
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    SpCas9 R691A mutant, Codon Optimized for mammalia…
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190141-plasmid-map-sequence-id-377886

    Enlarge

  12. pTox-EGFPsite1_NGA-PAM (BPK754)

    Plasmid
    #181747
    Purpose
    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM
    Depositor
    Benjamin Kleinstiver
    Publication
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
    Use
    Selection plasmid
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181747-plasmid-map-sequence-id-358237

    Enlarge

  13. PX459v2-ILK-gRNA 2-exon 8

    Plasmid
    #163321
    Purpose
    SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.
    Depositor
    Val Brunton
    Publication
    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ILK gRNA 2_Exon8
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6F
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only

  14. pLCHKO_SFRS7_exon_deletion_3

    Plasmid
    #155071
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155071-plasmid-map-sequence-id-308587

    Enlarge

  15. pLCHKO_MDM4_exon_deletion_3

    Plasmid
    #155075
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155075-plasmid-map-sequence-id-308578

    Enlarge

  16. pLCHKO_Ptbp_ex8_3

    Plasmid
    #155083
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155083-plasmid-map-sequence-id-308532

    Enlarge

Showing: 2181 - 2200 of 2672 results