We narrowed to 5,844 results for: pCas

1. pCAGGS-SMS1-TEV-Twin-Strep

   Plasmid

   #202528

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS1 (SGMS1 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TAA)

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

   Plasmid

   #107857

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 1) (TARDBP Human)
   

   Expression

   Mammalian

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA15-Ubc-NLS- scFV-tdTomato

   Plasmid

   #199446

   Purpose

   Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

   Depositor

   Baohui Chen

   Article

   Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

   Insert

   scFV-tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   human ubiquitin C (UBC)

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q

   Plasmid

   #172106

   Purpose

   Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)
   

   Expression

   Mammalian

   Mutation

   Gαq: C9S/C10S/Q209L

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mCherry-FKBP-GEF(ITSN1)

   Plasmid

   #222631

   Purpose

   Cdc42 activation using rapamycin

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   mCherry-FKBP-GEF(ITSN1) (ITSN1 Human)
   

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-ULK1 (836-1050)-MBP

   Plasmid

   #203562

   Purpose

   Mammalian expression of ULK1 (836-1050) with MBP tag

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ULK1 (ULK1 Human)
   

   Tags

   MBP-10His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-mouse PKA-Calpha-mEGFP

   Plasmid

   #45528

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA catalytic subunit alpha tagged by mEGFP
   

   Use

   With chicken beta actin promoter and wpre

   Expression

   Mammalian

   Available Since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS)

   Plasmid

   #137864

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSAS N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-Flag-hsDicer (Y971A/Y972A)

   Plasmid

   #41590

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y971A/Y972A

   Promoter

   CMV

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-Cas12i-Max-T2A-EGFP

    Plasmid

    #188268

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i-Max
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Cas12i-HiFi-T2A-EGFP

    Plasmid

    #188269

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i-HiFi
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid

    #174140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 SPASTIN , residues 112-196

    Plasmid

    #180627

    Purpose

    Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 112-196

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA528 SPASTIN , residues 108-200

    Plasmid

    #180628

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSD_N-SpCas9-1-573-FRB-1xNLS

    Plasmid

    #107298

    Purpose

    Expresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 1-573) fused to FRB
    

    Use

    CRISPR

    Tags

    FRB and SV40 NLS

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 1-573)

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-FLAG-SpCas9-HF1-plus

    Plasmid

    #126775

    Purpose

    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pspCas9-POLI-ST2-com vector

    Plasmid

    #176234

    Purpose

    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-FLAG-IRES-Blasticidin-hOCT4 WT

    Plasmid

    #198764

    Purpose

    Mammalian expression of hOCT4

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

    Insert

    hOCT4 WT (POU5F1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-rat PKA-RIIbeta-mEGFP

    Plasmid

    #45530

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII beta tagged by mEGFP
    

    Use

    With chicken beta actin promoter and wpre

    Expression

    Mammalian

    Available Since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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