We narrowed to 19,985 results for: aga

1. pX330_EZH2gRNA17

   Plasmid

   #199589

   Purpose

   Contains guide RNA to 3' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pS238D1::sptse5-CT

   Plasmid

   #192959

   Purpose

   For expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studies

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1317)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-IRES-GFP-human-caspase-1/4a

   Plasmid

   #183393

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domain of human caspase-1 fused to catalytic domain of human caspase-4 (CASP1 Synthetic, Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L2_2xNOP1gRNA-Cas9-CsA

   Plasmid

   #136140

   Purpose

   Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO.1P PRIM2_1

   Plasmid

   #160795

   Purpose

   Suppress PRIM2

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shPRIM2_1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMSCV-IRES-GFP-MD2 (F126A)-HA

   Plasmid

   #133067

   Purpose

   Retroviral expression of mouse MD2 (F126A) tagged with HA

   Depositor

   Jonathan Kagan

   Article

   Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

   Insert

   MD2 (F126A) (Ly96 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   F126A

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSCV-IRES-GFP-MD2 (C95A)-HA

   Plasmid

   #133066

   Purpose

   Retroviral expression of mouse MD2 (C95A) tagged with HA

   Depositor

   Jonathan Kagan

   Article

   Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

   Insert

   MD2 (C95A) (Ly96 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   C95A

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-CMV-TIRAP-cGASΔN-HA

   Plasmid

   #130921

   Purpose

   Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   TIRAP-ΔNcGAS
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cGAS N terminus (aa1-159) replaced with the N ter…

   Promoter

   CMV

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. gh268

   Plasmid

   #106867

   Purpose

   expression of gRNA targeting ALG14

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   ALG14 (ALG14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LRG-2.1T-neo-sgMYB(human)#5.2

    Plasmid

    #105592

    Purpose

    lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    gRNA targeting human MYB DBD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. WEE1 gRNA (BRDN0001148564)

    Plasmid

    #77369

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human WEE1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    WEE1 (WEE1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. N4BP2 gRNA (BRDN0001146240)

    Plasmid

    #76336

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human N4BP2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    N4BP2 (N4BP2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OBSCN gRNA (BRDN0001146767)

    Plasmid

    #76116

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human OBSCN

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    OBSCN (OBSCN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSicoR (EGFP) shPnky-2

    Plasmid

    #79142

    Purpose

    Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre

    Depositor

    Daniel Lim

    Article

    Ramos et al Cell Stem Cell. 2015 Apr 2;16(4):439-47.

    Insert

    shPnky-2 (Gm30731 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for the shRNA and CMV for EGFP

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCoHygro L16_shRNA of spliced Copia

    Plasmid

    #71389

    Purpose

    knock down expression of spliced Copia in Drosophila melanogaster

    Depositor

    Timothy Nilsen

    Insert

    Copia
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mU6-sgSik1_2nd-hU6-sgSik3_1st

    Plasmid

    #177218

    Purpose

    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Insert

    sgSik1_2nd/sgSik3_1st
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT2/shαSMA/GFP4_Seq2/clone1

    Plasmid

    #201402

    Purpose

    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    alpha-SMA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

    Plasmid

    #159130

    Purpose

    expresses BACH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCXLE-hOCT3/4-shp53-F

    Plasmid

    #27077

    Purpose

    Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. rAAV-U6-MeCP2shRNA-CamKIIa-mCherry

    Plasmid

    #169704

    Purpose

    MeCP2 Knockdown

    Depositor

    Ana Oliveira

    Article

    Gulmez Karaca et al Neurobiol Learn Mem. 2018 Mar;149:84-97.

    Insert

    shRNA against MeCp2 (Mecp2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge