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pTWIN1-His6-Ssp-Httex1-23Q-A2C-P90C

Plasmid

#114615

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 23 polyQ repeat

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q-A2C-P90C

Plasmid

#114615

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 23 polyQ repeat

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-A60C

Plasmid

#114628

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-A60C

Plasmid

#114628

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90-37Q-P90C

Plasmid

#114621

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 37 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90-37Q-P90C

Plasmid

#114621

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 37 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q-P80C

Plasmid

#114614

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 23 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q-P80C

Plasmid

#114614

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 23 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C

Plasmid

#114624

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C

Plasmid

#114624

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Httex1-43Q-A2C-P90C

Plasmid

#114625

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Httex1-43Q-A2C-P90C

Plasmid

#114625

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C

Plasmid

#114630

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C

Plasmid

#114630

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

Plasmid

#114610

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

Plasmid

#114610

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

Plasmid

#114609

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

Plasmid

#114609

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

Plasmid

#114620

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

Plasmid

#114620

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C

Plasmid

#114619

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C

Plasmid

#114619

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

Plasmid

#114618

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

Plasmid

#114618

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

Plasmid

#114608

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 15 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

Plasmid

#114608

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 15 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q

Plasmid

#114612

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 23 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q

Plasmid

#114612

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 23 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
DH10beta F' DOT sbcC (donor strain)

Bacterial Strain

#12082

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Bacterial Resistance

Tetracycline

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only
pET21b(+)-Is-PETase-W159H-S238F

Plasmid

#112203

Purpose

pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

Depositor

Gregg Beckham, Christopher Johnson

Article

Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

Insert

PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12

Tags

6XHIS

Expression

Bacterial

Mutation

W159H, S238F

Promoter

N/A

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge