We narrowed to 19,691 results for: ACE

1. pMZ103-rbs1-m1

   Plasmid

   #204463

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR A127N mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   LasR A127N mutation

   Promoter

   In an operon and PLas

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMZ103-rbs1-m2

   Plasmid

   #204464

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L128C mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   LasR L128C mutation

   Promoter

   In an operon and PLas

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMZ103-rbs1-m3

   Plasmid

   #204465

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127S mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   LasR L125W-A127S mutation

   Promoter

   In an operon and PLas

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMZ103-rbs1-m4

   Plasmid

   #204466

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   LasR L125W-A127T mutation

   Promoter

   In an operon and PLas

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMZ103-rbs1-m5

   Plasmid

   #204467

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125F-A127G-S129N mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   LasR L125F-A127G-S129N mutation

   Promoter

   In an operon and PLas

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMZ103-rbs1-m6

   Plasmid

   #204468

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-S129N-L130V mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   LasR L125W-S129N-L130V mutation

   Promoter

   In an operon and PLas

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMZ103-rbs1-m7

   Plasmid

   #204469

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T-L128M mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   LasR L125W-A127T-L128M mutation

   Promoter

   In an operon and PLas

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-PM-deltaCKAR

   Plasmid

   #222429

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Plasma membrane delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACYCDuet-1:ChrA

   Plasmid

   #225079

   Purpose

   Expresses precursor peptide ChrA in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrA
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET29_anti-SARS-CoV-2-NM1226

    Plasmid

    #209422

    Purpose

    Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-NM1226
    

    Use

    Tags

    Myc-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pET29_anti-SARS-CoV-2-NM1230

    Plasmid

    #209423

    Purpose

    Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-NM1230
    

    Use

    Tags

    Myc-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pET29_anti-SARS-CoV-2-Nanobody-C5

    Plasmid

    #209420

    Purpose

    Express anti-SARS-CoV-2-Nanobody C5 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-C5
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET29_anti-SARS-CoV-2-Nanobody-F2

    Plasmid

    #209421

    Purpose

    Express anti-SARS-CoV-2-Nanobody F2 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-F2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pACYCDuet-1:ChrA-C66S

    Plasmid

    #225081

    Purpose

    Expresses precursor peptide ChrA-C66S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C66S
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C66S

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHPHS927

    Plasmid

    #228238

    Purpose

    Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin reporter for integration into Bxb1 attP_GA landing pad

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

    Inserts

    mCherry
    Beta globin
    

    Use

    Tags

    3xFLAG, HA, and PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV and none

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHPHS800

    Plasmid

    #228240

    Purpose

    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

    Insert

    Cas9
    

    Use

    Tags

    Hygromycin phosphotransferase

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMRMTK-clo24

    Plasmid

    #203994

    Purpose

    Cloning plasmid containing the Hro ES5 insert with a chloramphenicol resistance marker

    Depositor

    Nathan Crook

    Article

    van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

    Insert

    Hro ES5
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Hro ES5

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Nuc-deltaCKAR

    Plasmid

    #222430

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Nucleus delta-selective C kinase activity reporter
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

    Plasmid

    #228508

    Purpose

    Bacterial Hybrid Driver Plasmid

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Insert

    T7 RNAP, LARP-I5CHO (#1)
    

    Use

    Synthetic Biology

    Tags

    Umu Degron, GTG-start codon

    Expression

    Mutation

    R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZB578_pB1H1_T7-RNAP_LARP-I

    Plasmid

    #228507

    Purpose

    Bacterial Hybrid Driver Plasmid

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Insert

    T7 RNAP, LARP-I
    

    Use

    Synthetic Biology

    Tags

    Umu Degron, GTG-start codon

    Expression

    Mutation

    R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. qTAG-AAVS1-PGK-Zeo

    Plasmid

    #227271

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX458-PLK4

    Plasmid

    #227310

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PLK4 (PLK4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSCD-cTEVP-RBD

    Plasmid

    #226233

    Purpose

    Express cTEVP-RBD fusion

    Depositor

    Michael Elowitz

    Article

    Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

    Insert

    cTEVP-RBD (TEVgp1 Tobacco etch virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSCD-nTEVP-RBD

    Plasmid

    #226232

    Purpose

    Express nTEVP-RBD fusion

    Depositor

    Michael Elowitz

    Article

    Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

    Insert

    nTEVP-RBD (TEVgp1 Tobacco etch virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSCD-HCVP

    Plasmid

    #226227

    Purpose

    Express HCVP

    Depositor

    Michael Elowitz

    Article

    Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

    Insert

    HCVP (POLY Hepatitis C virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. qTAG-C-mScarlet-EFS-Zeo

    Plasmid

    #227261

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-Golgi-deltaCKAR

    Plasmid

    #222428

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Golgi delta-selective C kinase activity reporter
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUt-MONARCH 2.0_crEGFP

    Plasmid

    #224793

    Purpose

    LPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Inserts

    crEGFP
    Triplex stablier
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-d2i

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC-hU6-2O-crScaffold_SV40-BFP

    Plasmid

    #224861

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC-hU6-2O-crScaffold_EF1a-BFP

    Plasmid

    #224862

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pACYCDuet-1:ChrA-C63S

    Plasmid

    #225080

    Purpose

    Expresses precursor peptide ChrA-C63S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C63S
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C63S

    Promoter

    T7

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a:ChrH

    Plasmid

    #225084

    Purpose

    Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrH
    

    Use

    Tags

    His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a:ChrI

    Plasmid

    #225085

    Purpose

    Expresses anthrone-oxygenase like enzyme ChrI in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrI
    

    Use

    Tags

    His6 and His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225062

    Purpose

    Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225063

    Purpose

    Expression of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBa.PEX 1-42-tdTM-FKBP

    Plasmid

    #224415

    Purpose

    Mammalian expression of Pex3

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    Pex3 (Pex3 Mouse)
    

    Use

    Tags

    tdTom-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLPP4

    Plasmid

    #209966

    Purpose

    Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P40)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLP-EV

    Plasmid

    #209973

    Purpose

    Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    CDS (mock)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SL3-MS2-gRNA GG backbone (MOBE3-4)

    Plasmid

    #219949

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-SL3 (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 3'-com-gRNA GG backbone (MOBE1-4)

    Plasmid

    #219947

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-com-3'end (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge