We narrowed to 7,789 results for: sms

1. pEK20

   Plasmid

   #210582

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   BdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Promoter

   BdGAPDHpro

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a-His6-SUMO-VAMP2

   Plasmid

   #135551

   Purpose

   Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP2
   

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA Raptor mutant 9

   Plasmid

   #8476

   Depositor

   David Sabatini

   Article

   Kim et al Cell 2002 Jul 26;110(2):163-75.

   Insert

   Raptor (RPTOR Human)
   

   Tags

   ha

   Expression

   Mammalian

   Mutation

   1191RVYDRR1196-DAAADD (R1191D, V1192A, Y1193A, D1…

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

4. Nup54 no FG-mEGFP494(0)

   Plasmid

   #164269

   Purpose

   Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP

   Depositor

   Sanford Simon

   Article

   Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

   Insert

   Nup54 (NUP54 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   deleted the FG region of Nup54

   Promoter

   CMV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. YFP-Rpp20-C1

   Plasmid

   #134541

   Purpose

   Expresses YFP-Rpp20 fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   Rpp20
   

   Tags

   YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-GFPIvy-SpHis5

   Plasmid

   #60783

   Purpose

   Yeast C-terminal tagging vector, which creates gene fusions with GFPIvy using SpHis5 selection

   Depositor

   Linda Huang

   Article

   Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

   Insert

   GFPIvy
   

   Use

   Yeast integrating

   Mutation

   S30R Y39N T65C Q69A S72A F99S N105T Y145F M153T V…

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBP-Bba_K822000

   Plasmid

   #74094

   Purpose

   L-Lactate inducible promoter from E. coli MG1655

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Bba_K822000 (L-Lactate promoter - E. coli MG1655)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   NA

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-dH3-YFP

   Plasmid

   #8694

   Depositor

   Kami Ahmad

   Article

   Janicki et al Cell 2004 Mar 5;116(5):683-98.

   Insert

   H3 (His3:CG31613 Fly)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   June 30, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV5-Fyn C3,6S

   Plasmid

   #19979

   Depositor

   Marilyn Resh

   Article

   Alland et al J Biol Chem. 1994 Jun 17. 269(24):16701-

   Insert

   Fyn (FYN Human)
   

   Expression

   Mammalian

   Mutation

   C3,6S

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pBS NL4-3 envFS eGFP Δgag/pol

    Plasmid

    #101343

    Purpose

    Deletion from the start of gag until 229 bases before the cPPT

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Mutation

    delta GagPol

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNC1137

    Plasmid

    #41563

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9
    

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bov.hsc70.T77.A1-386.wt

    Plasmid

    #12534

    Depositor

    David McKay

    Article

    Wilbanks et al J Biol Chem. 1994 Apr 29. 269(17):12893-

    Insert

    Bovine 70 kDa heat shock protein ATPase fragment
    

    Expression

    Bacterial

    Mutation

    deletion of residues 387-650 of bovine Hsc70 prot…

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiGuide_mCherry_sgEPAS1#2

    Plasmid

    #183123

    Purpose

    pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgEPAS1-2
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBP-pET-RBS

    Plasmid

    #72981

    Purpose

    PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    pET RBS derived from pET vectors
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-PtH

    Plasmid

    #133391

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIE2-AD

    Plasmid

    #21603

    Depositor

    Takahiro Kusakabe

    Article

    Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

    Insert

    NF-kappa-B p65 subunit (Rela Mouse)
    

    Use

    Gateway destination vector

    Tags

    Flag, Gateway destination cassette, and NLS

    Expression

    Insect

    Available Since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a-His6-SUMO-VAMP8

    Plasmid

    #135553

    Purpose

    Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP8
    

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMP56-5

    Plasmid

    #45904

    Depositor

    Harmit Malik

    Article

    Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

    Insert

    mitochondrial antiviral signaling protein, mutation C508R (MAVS Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C508R

    Promoter

    CMV

    Available Since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

19. BII-C3HTK

    Plasmid

    #133388

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. nonphosphorylatable Cenp-B-targted Aurora B FRET sensor

    Plasmid

    #45230

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    nonphos Cenp-B targeted Aurora B FRET sensor
    

    Expression

    Mammalian

    Mutation

    t-a mutation in substrate sequence

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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