We narrowed to 3,228 results for: zebrafish

1. pDestTol2CG2-mnx1:ReaChR-citrine

   Plasmid

   #169046

   Purpose

   Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   ReaChR-Citrine
   

   Use

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   Promoter

   3xMnx1

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2+-FLAG3-drAgo2DY-EF

   Plasmid

   #221716

   Purpose

   Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2DY-EF (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   D651E and Y680F in active site

   Promoter

   simian CMV IE94

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEM-NLS-Cre-2A-mCherry-FKF

   Plasmid

   #119868

   Purpose

   Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. E1b-GFP-Tol2-Gateway

   Plasmid

   #37846

   Depositor

   Nadav Ahituv

   Article

   Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

   Type

   Empty backbone

   Use

   Zebrafish enhancer assay vector

   Tags

   Expression

   Mutation

   Promoter

   E1b

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS2TAL3-DD

   Plasmid

   #37275

   Purpose

   TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

   Depositor

   David Grunwald

   Article

   Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

   Type

   Empty backbone

   Use

   Tags

   Flag, Fok I DD, Tal-C, and Tal-N

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. actc1b-2PH-PLCd-mKate2

   Plasmid

   #109500

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-2PH-PLCd-mKate2
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. actc1b-mKate2-ING2

   Plasmid

   #109502

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-mKate2-ING2
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGTag-P2A-QFGal4-SV40pA

   Plasmid

   #155127

   Purpose

   Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   P2A-QFGal4
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV_PP1-mCitrine

   Plasmid

   #172472

   Purpose

   Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

   Depositor

   Sean R Collins

   Article

   Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

   Insert

   prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
   

   Use

   Lentiviral

   Tags

   mCitrine and prolactin signal sequence (cleaved p…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p5E-6xgata2aECEbasp

    Plasmid

    #132968

    Purpose

    gata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    gata2a endothelial enhancer (x6) with a b-actin basal promoter
    

    Use

    Gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Tol2-LyzC-GFP-U6ac-ctrl-guides

    Plasmid

    #168240

    Purpose

    "neutrophil specific GFP with ubiquitous ctrl sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    control sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTransgenesis p3 sv40 minimal promoter:: Katushka RFP

    Plasmid

    #131731

    Purpose

    Transposon based transgenesis

    Depositor

    Rene Maehr

    Article

    Stanney W et al Dev Biol. 2020 Mar 15;459(2):161-180. do

    Insert

    SV40MinPromoter
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR_PGK_antiCD19_Z3_Gal4VP64

    Plasmid

    #169916

    Purpose

    Expression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.

    Depositor

    Zhili Rong

    Article

    Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

    Insert

    antiCD19-Notch3-GL4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT2-cryR;dll4P1Egfp

    Plasmid

    #90160

    Purpose

    contains dll4 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    dll4P1Egfp (dll4 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PUC57_BAG3P209L-eGFP

    Plasmid

    #98183

    Purpose

    Codes for full length, GFP tagged mutant Bag3

    Depositor

    Robert Bryson-Richardson

    Article

    Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

    Insert

    BAG3P209L-eGFP (BAG3 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    P209L

    Promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT2-cryR;dll4CE1-P1Egfp

    Plasmid

    #90146

    Purpose

    contains dll4 enhancer 1 sequence, and dll4 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    dll4E1P1Egfp (dll4 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT2-cryR;lmo2CE1-P1Egfp

    Plasmid

    #90153

    Purpose

    contains lmo2 enhancer sequence, and lmo2 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    lmo2E1P1Egfp (lmo2 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT2-cryR;lmo2P1Egfp

    Plasmid

    #90162

    Purpose

    contains lmo2 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    lmo2P1Egfp (lmo2 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 782 pMEActMEK

    Plasmid

    #31166

    Depositor

    Nathan Lawson

    Article

    Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

    Insert

    MEK (MAP2K1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Ser218Glu Ser222Asp; activated MEKK

    Promoter

    Available since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

20. 1352wUV2 F2bbs kan GCANNNAGGk

    Plasmid

    #18752

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Promoter

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge