We narrowed to 162,449 results for: addgene

1. PNCA-GFP

   Plasmid

   #67789

   Purpose

   Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

   Insert

   MMLV-LTR-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLV-mRFP1-Human UTROPHIN_1-261

   Plasmid

   #225052

   Purpose

   Express in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.

   Depositor

   Pantelis Tsoulfas

   Insert

   Human Utrophin residues 1-261 and RFP1 (UTRN Human, Homo Sapiens)
   

   Use

   Lentiviral

   Tags

   Human UTROPHIN 1-261 and mRFP1

   Expression

   Mammalian

   Mutation

   Sequence is derived from ADDGENE Plasmid #26739-R…

   Promoter

   CMV promoter and enhancer

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EGFP-Human-UTROPHIN_1-261

   Plasmid

   #222393

   Purpose

   Express in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.

   Depositor

   Pantelis Tsoulfas

   Insert

   Human Utrophin residues 1-261 and EGFP (UTRN Human, Homo Sapiens)
   

   Use

   Lentiviral

   Tags

   EGFP and Human UTROPHIN 1-261

   Expression

   Mammalian

   Mutation

   Sequence is derived from ADDGENE Plasmid #26737-G…

   Promoter

   CMV promoter and enhancer

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. DNA-PKcs N-terminal sgRNA

   Plasmid

   #207093

   Purpose

   pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   AGCGGGACTCGGCGGCATGG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CarO-BFP2-TSERex

   Plasmid

   #124795

   Purpose

   Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

   Depositor

   Yulong Li

   Article

   Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

   Insert

   CarO-BFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Mac-BFP2-TSERex

   Plasmid

   #124793

   Purpose

   Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

   Depositor

   Yulong Li

   Article

   Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

   Insert

   Mac-BFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MCP-EGFP-PiggyBac

   Plasmid

   #190003

   Purpose

   Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

   Depositor

   Tineke Lenstra

   Article

   Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

   Insert

   MCP-EGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-NED

   Plasmid

   #109358

   Purpose

   Mammalian vector for expressing effector domains fused to dCas9.

   Depositor

   Antal Kiss

   Article

   Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   dCas9, SV40 NLS, 3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-PGK-CXCR4 (neo)

    Plasmid

    #192077

    Purpose

    Lentivirus for expression of CXCR4

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ERoxBFP

    Plasmid

    #68126

    Purpose

    mammalian expression of ER localized oxBFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Insert

    oxBFP
    

    Use

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198048

    Purpose

    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EdTC

    Plasmid

    #24310

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-dnhTcf4 // SV40-mCherry (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    31 first amino acids of hTcf4 have been deleted.

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. ATG9A sgRNA

    Plasmid

    #207557

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CACTGAATACCAGCGCCTAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. ULK1 sgRNA

    Plasmid

    #207559

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CCAGCCAGGCCAGAAAGGTC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. TALEN-mROSA26 ELD

    Plasmid

    #60026

    Purpose

    Expression of a TALEN protein specific for mouse ROSA26 locus.

    Depositor

    Radislav Sedláček

    Article

    Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

    Insert

    TALEN-ROSA26
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. 7TG

    Plasmid

    #24314

    Depositor

    Roel Nusse

    Article

    Brugmann et al Development. 2007 Sep . 134(18):3283-95.

    Insert

    7xTcf-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. WIPI2 sgRNA

    Plasmid

    #207554

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the WIPI2 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CGCGCGCCCAGCCATGAACC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF-I-GFP GX

    Plasmid

    #45443

    Depositor

    John Brigande

    Article

    Gubbels et al Nature. 2008 Sep 25. 455(7212):537-41.

    Type

    Empty backbone

    Use

    Tags

    IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1α

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pX459_gRNA-AAVS1_hspCas9

    Plasmid

    #193309

    Purpose

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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