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pTwist Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-HDR-donor

Plasmid

#221839

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoforms BFH) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pACUH 5-HT2BR-7xGFP11-HA

Plasmid

#221840

Purpose

Plasmid for generating 10xUAS-5-HT2BR-7xGFP11-HA transgenic flies with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

5-HT2BR (CG42796 Fly)

Tags

7xGFP11-HA

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[1-20]-N-int

Plasmid

#199210

Purpose

For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein Gal4[1-20]-N-int

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[21-881]-C-int

Plasmid

#199211

Purpose

For ubiquitous expression of split-intein Gal4-C-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHM148

Plasmid

#225532

Purpose

Encodes constitutive rtTA, tetOn-Pcdh19-GFP, and constitutive mCherry

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

rtTA
Pcdh19 (Pcdh19 Mouse)
mCherry

Tags

GFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSN539

Plasmid

#226775

Purpose

Expresses Drosophila UNC-104(wild-type) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKC51

Plasmid

#226779

Purpose

Expresses Drosophila UNC-104(A255V) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (A255V) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Alanine 255 to Valine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Nanos (HK65)

Plasmid

#206468

Purpose

Nanos expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Nanos (nos Fly)

Tags

PH mcherry

Expression

Insect

Mutation

M24E

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Bam M24E (HK88)

Plasmid

#206467

Purpose

Bam expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Bam (bam Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Tudor isoform A (HK130)

Plasmid

#206465

Purpose

Tudor expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Tudor (tud Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-NOT3 isoform A (HK100)

Plasmid

#206457

Purpose

NOT3 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT3 (Not3 Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-λN-HA-NOT1 isoform C (JM155)

Plasmid

#206449

Purpose

Not1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Not1 (Not1 Fly)

Tags

lambda N HA

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mCherry-Nanos isoform B (JM154)

Plasmid

#206447

Purpose

Nanos expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Nanos (nos Fly)

Tags

mCherry

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-NOT1 isoform C (HK53)

Plasmid

#206440

Purpose

NOT1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT1 (Not1 Fly)

Tags

mEGFP

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-CCR4 isoform A (HK52)

Plasmid

#206439

Purpose

CCR4 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

CCR4 (twin Fly)

Tags

mEGFP

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-NOT2 isoform A (HK54)

Plasmid

#206441

Purpose

NOT1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT1 (Not1 Fly)

Tags

mEGFP

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-NOT3 isoform A (HK55)

Plasmid

#206442

Purpose

NOT3 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT3 (Not3 Fly)

Tags

mEGFP

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mEGFP-Cup isoform B (JM051)

Plasmid

#206451

Purpose

Cup expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Cup (cup Fly)

Tags

PH mEGFP

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-CAF1 isoform A (HK96)

Plasmid

#206454

Purpose

CAF1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

CAF1 (Pop2 Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-NOT2 isoform A (HK99)

Plasmid

#206456

Purpose

NOT2 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT2 (Rga Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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