We narrowed to 162,449 results for: addgene

1. pLenti UBC hNLRP3-mNG

   Plasmid

   #218642

   Purpose

   Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVPRT-tTR-KRAB

   Plasmid

   #11648

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hPrion, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv2-Stuffer-HepmCherry

   Plasmid

   #192825

   Purpose

   Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNR64

   Plasmid

   #79540

   Purpose

   Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   TP901
   BxbI
   

   Use

   Tags

   His tag and LAA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. VPS35 shRNA2

   Plasmid

   #163859

   Purpose

   shRNA targeting the coding sequences of VPS35

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   VPS35 retromer complex component (VPS35 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. VV073: 1xCox8 - GCaMP5G in fubi

   Plasmid

   #58509

   Purpose

   Expresses the calcium sensor GCaMP5G in the mitochondria

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   1xCox8 - GCaMP5G
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6BHD (SETDB1)

   Plasmid

   #119955

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   SETDB1 (SETDB1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 190-410

   Promoter

   T7

   Available since

   Feb. 1, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pL1P5 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198046

   Purpose

   MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. ICP8-P2A/Crimson (pSLIK4)

   Plasmid

   #113859

   Purpose

   For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   ICP8
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Expression

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. p8389 LentiCRISPRv2 Neo sgNT-1

    Plasmid

    #221649

    Purpose

    Expression of non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    non-targeting sgNT-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSNAPf-Nup43

    Plasmid

    #98276

    Purpose

    mammalian expression of SNAPf-Nup43

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    Nup43 (NUP43 Human)
    

    Use

    Tags

    SNAPf-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MXS_CMV::PuroR-bGHpA

    Plasmid

    #62439

    Purpose

    MXS_chaining vector with CMV::PuroR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Puromycin with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiMutB3GNT5-blast

    Plasmid

    #208380

    Purpose

    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nt 922 c->g (PAM site inactivation)

    Promoter

    EF-1 alpha core promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ATG16L1 sgRNA

    Plasmid

    #207563

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    GCAGCAAGTGACATGTCGTC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHPyV7-713a

    Plasmid

    #24728

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    Full genome of Human polyomavirus 7
    

    Use

    Viral clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. p184_LTJ_sgRNACD90.2

    Plasmid

    #82670

    Purpose

    sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD90.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIgnaviCas9

    Plasmid

    #127595

    Purpose

    Expresses IgnaviCas9 in E. coli

    Depositor

    Stephen Quake

    Article

    Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

    Insert

    IgnaviCas9
    

    Use

    Tags

    6xHis Tag and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. 53BP1 N-terminal sgRNA

    Plasmid

    #207091

    Purpose

    pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GGGGAGCAGATGGACCCTAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEVY-T

    Plasmid

    #51232

    Purpose

    bi-directional expression vector for yeast, constitutively active, TRP1 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD/ADH1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHPyV6-607a

    Plasmid

    #24727

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    Full genome of Human polyomavirus 6
    

    Use

    Viral clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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