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pLKO.1-puro-shFAM134B

Plasmid

#109013

Purpose

short-hairpin knockdown of targeted gene

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

shFAM134B (RETREG1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGH224_sgRNA_2xMS2_Puro

Plasmid

#85413

Purpose

hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDIRE

Plasmid

#26745

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

iCre expression cassette

Use

Cre/Lox; Flp/frt

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pIgnaviCas9

Plasmid

#127595

Purpose

Expresses IgnaviCas9 in E. coli

Depositor

Stephen Quake

Article

Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

Insert

IgnaviCas9

Tags

6xHis Tag and Maltose binding protein

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLVPT-tTR-KRAB

Plasmid

#11642

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pLVET-tTR-KRAB

Plasmid

#11644

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hEF-1alpha, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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Lys-eMagA-EGFP

Plasmid

#162246

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

p18-eMagA-EGFP

Expression

Mammalian

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1-GFP

Plasmid

#180420

Purpose

Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.

Depositor

Nancy Moran

Article

Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

GFP

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-EF1a-Puro

Plasmid

#127170

Purpose

PCR template for EF1a-puro resistance cassette

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

None

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBHM2681

Plasmid

#241726

Purpose

Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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6BHD (SETDB1)

Plasmid

#119955

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

SETDB1 (SETDB1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

contains amino acids 190-410

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMIG-Aalpha WT

Plasmid

#10884

Depositor

William Hahn

Article

Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

Insert

PP2A Aalpha (PPP2R1A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pTK229

Plasmid

#71256

Purpose

luciferase reposter with 229 bp of the HSV TK promoter

Depositor

Peter Cockerill

Article

Hawwari et al J Immunol. 2002 Aug 15;169(4):1876-86.

Insert

Thymidine Kinase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

HSV Thymidine Kinase

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pKL10-mRuby-iATPSnFR1.0 bb118

Plasmid

#158980

Purpose

Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

mruby-iATPSNfR1.0

Tags

mruby3

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#102557

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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p-dCas9-LSD1-Hygro

Plasmid

#104406

Purpose

transient expression of dCas9-LSD1 fusion protein

Depositor

Stephan Beck

Insert

LSD1 (KDM1A Human)

Use

CRISPR

Expression

Mammalian

Mutation

IRATp970A0364D (Source Bioscience) has a P405H ch…

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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miniCMV-iRFP670-24xMS2

Plasmid

#238915

Purpose

Contains miniCMV promoter expressing iRFP670 mRNA taged 24xMS2 motif

Depositor

Jian-Hui Jiang

Article

Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

Insert

iRFP670-24xMS2

Use

Lentiviral

Expression

Mammalian

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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7TG

Plasmid

#24314

Depositor

Roel Nusse

Article

Brugmann et al Development. 2007 Sep . 134(18):3283-95.

Insert

7xTcf-eGFP

Use

Lentiviral

Mutation

EGFP is GFP with S65T and F64L mutations

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pBEVY-U

Plasmid

#51230

Purpose

bi-directional expression vector for yeast, constitutively active, URA3 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GPD/ADH1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pWZL hygro 12S E1A

Plasmid

#18748

Depositor

Scott Lowe

Article

Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Insert

12S E1A

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo

Plasmid

#153422

Purpose

MSCV with IRES-Neomycin resistant gene

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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