We narrowed to 4,520 results for: Alk

1. pET28b-DiCas7-11-His

   Plasmid

   #234482

   Purpose

   Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   DiCas7-11
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458_mCherry-EWSR1-gRNA

   Plasmid

   #237683

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to EWSR1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EWSR1 gRNA (EWSR1 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-MEKDD

   Plasmid

   #31202

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   MAPKK1 (MAP2K1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   S218D S222D

   Promoter

   Available since

   June 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5_mEGFP-SS18-SSX2

   Plasmid

   #237637

   Purpose

   For overexpression of mEGFP-SS18-SSX2

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   SS18-SSX2 (SS18, SSX2 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR-XL-rAkap9

   Plasmid

   #196867

   Purpose

   Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akap9 (Akap9 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac promoter and T7 promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. hSyn-GtACR2-ChRmine-tandem-eYFP

   Plasmid

   #192580

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   EYFP- soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-V3-CD20-Puro

   Plasmid

   #209757

   Purpose

   Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LCV2_mCherry_AAVS1_sgRNA_2

   Plasmid

   #155105

   Purpose

   lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER22-MKK6E-Flag

   Plasmid

   #99259

   Purpose

   Lentiviral vector encoding a doxycycline-inducible Flag-tagged constitutively active MKK6.

   Depositor

   Alejandro Adam, Peter Vincent

   Article

   Adam et al PLoS One. 2016 Sep 7;11(9):e0161975. doi

   Insert

   MKK6E (MAP2K6 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S207E T211E

   Promoter

   TRE2

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDY0207 ACTB atgRNA with v1 scaffold

    Plasmid

    #179107

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-hRedOpsin-fCX3CL1

    Plasmid

    #164421

    Purpose

    AAV plasmid expressing full-length CX3CL1 (fractalkine) in photoreceptors

    Depositor

    Connie Cepko

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

    Insert

    Cx3cl1 (Cx3cl1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human red opsin

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDY0310 TP901-1 Integrase

    Plasmid

    #179110

    Purpose

    TP901-1 Integrase expression vector

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    TP901-1 Integrase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCKO_AAVS1_sgRNA_2

    Plasmid

    #155085

    Purpose

    lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBactin-Nedd1-mOrange2-hCM1

    Plasmid

    #196863

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-hCM1 (Nedd1 Human, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-CB1-HIF2a-GFP-T2A-Puro

    Plasmid

    #71708

    Purpose

    Lentiviral expression of HIF2A-GFP

    Depositor

    Eric Jonasch, Xiande Liu

    Article

    Liu et al Oncogene. 2015 May 7;34(19):2450-60. doi

    Insert

    HIF2A (EPAS1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. B2M Bulldozer (gRNA crB2M_13)

    Plasmid

    #84381

    Purpose

    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells

    Depositor

    Chad Cowan

    Article

    Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.

    Insert

    B2M Bulldozer crB2M_13 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223-H-RAS V12

    Plasmid

    #31201

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    H-Ras (HRAS Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    G12V constitutively active

    Promoter

    Available since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

    Plasmid

    #196890

    Purpose

    Neuron-specific expression of human (h)Centrin fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen-hCentrin1 (CETN1 Human)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-Best1-sCX3CL1

    Plasmid

    #164422

    Purpose

    AAV plasmid expressing soluble CX3CL1 (fractalkine) in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

    Insert

    Cx3cl1 (Cx3cl1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    deleted terminal 59 amino acids

    Promoter

    Best1

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

    Plasmid

    #161923

    Purpose

    To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    sgRNA targeting HSD17B11 exon 1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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