We narrowed to 3,118 results for: GAI

1. pMZ285

   Plasmid

   #52226

   Purpose

   Expression of sgRNA precursor targeted against ade6M210

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Insert

   rrk1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   rrk1

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NFATc4-CRISPR-gRNA#exon5-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124290

   Purpose

   sgRNA against huiman NFATc4

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   NFATc4 nuclear factor of activated T cells (NFATC4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

3. Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124283

   Purpose

   sgRNA against human Thy-1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

4. Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124285

   Purpose

   sgRNA against human Thy-1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

5. Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124284

   Purpose

   sgRNA against human Thy-1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

6. NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124289

   Purpose

   sgRNA against human NFATc4

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   NFATc4 nuclear factor of activated T cells (NFATC4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

7. pMZ286

   Plasmid

   #52227

   Purpose

   Expression of sgRNA precursor targeted against ade6L469

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Insert

   rrk1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   rrk1

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR U6-hTERTsh

   Plasmid

   #111385

   Purpose

   Knock down of hTERT transcript

   Depositor

   Klaus Holzmann

   Article

   Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

   Insert

   shRNA C3 against hTERT
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSB700-mouse-ACTC1-Puro

   Plasmid

   #128326

   Purpose

   SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

   Depositor

   Alejandro Chavez

   Insert

   gRNA against mouse ACTC1 for activation (Actc1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid

    #118158

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mSprouty1/3B14

    Plasmid

    #22091

    Depositor

    Gail Martin

    Article

    Minowada et al Development. 1999 Oct . 126(20):4465-75.

    Insert

    Sprouty 1 (Spry1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

12. 3ME6/mSprouty2

    Plasmid

    #22097

    Depositor

    Gail Martin

    Article

    Minowada et al Development. 1999 Oct . 126(20):4465-75.

    Insert

    Sprouty 2 (Spry2 Mouse)
    

    Use

    Bacterial

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pTALYM4SpMi-01

    Plasmid

    #47879

    Purpose

    TALE-mRuby2 against Mus spretus minor satellite sequence containing two SNPs

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. cFGF8

    Plasmid

    #22089

    Depositor

    Gail Martin

    Article

    Crossley et al Cell. 1996 Jan 12. 84(1):127-36.

    Insert

    FGF8 (FGF8 Chicken)
    

    Use

    Bacterial

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

15. PCTK1

    Plasmid

    #39053

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PCTK1 (CDK16 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Activation loop mutant S319D

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pGKO1

    Plasmid

    #63611

    Purpose

    a binary Agrobacterium vector carrying the HSVtk gene as a negative selection marker against ectopic transformants.

    Depositor

    Seogchan Kang

    Article

    Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

    Type

    Empty backbone

    Use

    Binary fungal vector

    Tags

    Expression

    Mutation

    Promoter

    Lac Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLfa PUb TAL-KMO-R

    Plasmid

    #52888

    Purpose

    Right hand TALEN against Aedes aegypti KMO gene

    Depositor

    Zach Adelman

    Article

    Aryan et al PLoS One. 2013;8(3):e60082. doi: 10.1371

    Insert

    TALEN-KMO
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ae aegypti polyubiquitin

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124288

    Purpose

    sgRNA against human NFATc3

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    NFATc3 nuclear factor of activated T cells (NFATC3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pKG136

    Plasmid

    #63075

    Purpose

    Retroviral expression of EGFP-TEV-S-tag CENP-W for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    CENP-W (CENPW Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    E. coli codon optimized, hardened against Dharmac…

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3CB1/cSprouty2

    Plasmid

    #22096

    Depositor

    Gail Martin

    Article

    Minowada et al Development. 1999 Oct . 126(20):4465-75.

    Insert

    Sprouty 2 (SPRY2 Chicken)
    

    Use

    Bacterial

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only