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We narrowed to 342 results for: GAI

Showing: 1 - 20 of 342 results

  1. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...mentor others more successfully. But how does one gain such experience? Your institution may offer occasional... leadership training began with the NPA where I gained invaluable experience working on and later chairing... 5 years and just recently stepped down. I have gained invaluable experience and even after 5 years, was... of the NPA’s website. By volunteering, you can gain experience working with other people mostly online... Opening doors to further opportunities  Having gained leadership skills and advocacy experience with ... students to think about their career options, gaining transferable skills such as leadership skills can...

  2. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...for generating sybodies against membrane proteins Developing antibodies against proteins embedded in membranes...camelid animal facility which can be expensive to gain access to routinely, and it can be especially challenging...challenging when trying to develop antibodies against unstable targets like membrane proteins that may...plasmids to generate sybodies, synthetic nanobodies against the spike protein (Walter et al., 2020). No llamas...selection of synthetic nanobodies, called sybodies, against membrane protein targets in as little as 3 weeks...Outline of the process for selection of sybodies against membrane proteins. Image from Zimmermann et al....Seeger MA (2020) Generation of synthetic nanobodies against delicate proteins. Nature Protocols 15:1707–1741...

  4. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...efforts. So, how effective are the current vaccines against the variants? Alejandro Balazs, a Group Leader...study. Figure 1: Neutralization levels against spike pseudovirus decreases depending on the variant...as the target of vaccines and allows the virus to gain entry into human cells via human ACE2 receptors....studies on the effectiveness of current vaccines against SARS-CoV-2 variants, this study was the first to...

  6. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...10.1093/hmg/10.19.2109 Sandrin V, Boson B, Salmon P, Gay W, Nègre D, Le Grand R, Trono D, Cosset F-L (2002...

  7. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected ...indirect Enzyme-Linked ImmunoSorbent Assay (ELISA) against a purified antigen to validate an antibody. This... the antigen and how to probe with an antibody against the target to show a dose response. Sharing speeds...minutes. Absorbance was read at 450 nm and plotted against the micrograms of antigen loaded. Last reviewed...

  8. Antibody Guide

    Type
    Collection
    ...developed, the antibodies are collected, tested against the antigen, packaged, and sold. This is the most...unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving allergic... directly to the sdAb, or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs...most or all of their extracellular matrix removed. Again, the signaling molecule may be a fluorophore or ...or more conjugated primary antibodies, and wash again. Use controls to identify, or gate, cells by size... validated for a similar assay type) and search again. After filtering, narrow down further by company... a new antibody using the above process and try again. In some cases, it may be preferable to buy small...

  9. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies A panel of recombinant monoclonal antibodies against zebrafish neural receptors and secreted proteins...Toolbox for making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain...May 24;7. and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus...

  10. p53 Pathway

    Type
    Collection
    ...impacts of p53 mutations have been characterized as gain-of-function, expanding the mechanisms by which mutant...OTT.S53876. PubMed PMID: 24379683 . When mutants gain new powers: news from the mutant p53 field. Brosh...89(7):1175-84. PubMed PMID: 9215639 . Mutant p53 gain-of-function in cancer. Oren, M. and V. Rotter. Cold...

  11. Immunocytochemistry

    Type
    Protocol
    ...staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody...labeling cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody...

  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving to be...genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007. Scientists...Horvath P. 2007. CRISPR provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709...

  13. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...careers to participate in the community. MCC members gain access to mentors, virtual and local professional...External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP...

  14. Rett Syndrome

    Type
    Collection
    ...repetitive hand stereotypies, and gait dysfunction or absence of gait. Greater than 95% of individuals ...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Curr Biol. 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean...polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-...Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu Nagai Tension TS module, Vinculin tension sensors Measuring...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...easily identify your protein using an antibody against that epitope. This could allow you to conduct...favorite gene even if you do not have an antibody against it. Another common scenario is fusing your...

  17. Zhang Lab CRISPR Page

    Type
    Collection
    ... microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial...isoforms form the RefSeq database (23,430 isoforms) for gain-of-function screening. For more information, please...
Showing: 1 - 20 of 342 results