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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7 results for: GAI

Showing: 1 - 7 of 7 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected ...indirect Enzyme-Linked ImmunoSorbent Assay (ELISA) against a purified antigen to validate an antibody. This... the antigen and how to probe with an antibody against the target to show a dose response. Sharing speeds...minutes. Absorbance was read at 450 nm and plotted against the micrograms of antigen loaded. Last reviewed...

  2. Immunocytochemistry

    Type
    Protocol
    ...staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody...labeling cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody...

  5. Pouring LB Agar Plates

    Type
    Protocol
    ...re-liquefy the agar by running it through the autoclave again or by microwaving (if you microwave, beware of over-boiling...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...modified for other targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit ...
Showing: 1 - 7 of 7 results