We narrowed to 303 results for: GAI
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TypeBlog PostPublishedAug. 6, 2015, 2:30 p.m....mentor others more successfully. But how does one gain such experience? Your institution may offer occasional... leadership training began with the NPA where I gained invaluable experience working on and later chairing... 5 years and just recently stepped down. I have gained invaluable experience and even after 5 years, was... of the NPA’s website. By volunteering, you can gain experience working with other people mostly online... Opening doors to further opportunities Having gained leadership skills and advocacy experience with ... students to think about their career options, gaining transferable skills such as leadership skills can...
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No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m....for generating sybodies against membrane proteins Developing antibodies against proteins embedded in membranes...camelid animal facility which can be expensive to gain access to routinely, and it can be especially challenging...challenging when trying to develop antibodies against unstable targets like membrane proteins that may...plasmids to generate sybodies, synthetic nanobodies against the spike protein (Walter et al., 2020). No llamas...selection of synthetic nanobodies, called sybodies, against membrane protein targets in as little as 3 weeks...Outline of the process for selection of sybodies against membrane proteins. Image from Zimmermann et al....Seeger MA (2020) Generation of synthetic nanobodies against delicate proteins. Nature Protocols 15:1707–1741... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m. -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....efforts. So, how effective are the current vaccines against the variants? Alejandro Balazs, a Group Leader...study. Figure 1: Neutralization levels against spike pseudovirus decreases depending on the variant...as the target of vaccines and allows the virus to gain entry into human cells via human ACE2 receptors....studies on the effectiveness of current vaccines against SARS-CoV-2 variants, this study was the first to... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....Neela, P.H., Zarnegar, B.J., Mondal, S., Roth, J.G., Gai, H., Kovalski, J.R., Siprashvili, Z., Palmer, T.D... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....10.1093/hmg/10.19.2109 Sandrin V, Boson B, Salmon P, Gay W, Nègre D, Le Grand R, Trono D, Cosset F-L (2002... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m.... received a lot of attention on social media. I gained a concrete example to use to promote future attempts...it. I am able to write about any topic I like and gain experience in communication, editing, marketing,...online. Twitter is an excellent resource that has gained a lot of traction with researchers. It is an excellent...professional organizations, and other nonprofits to gain support for CINSO. For instance, I tweeted to the...volunteering in the “Dinosphere” almost every week. I gained experience explaining science to visitors of all... still do too! Beyond the outreach experience I gained from volunteering, it gave me a place to go once... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....antimicrobials could become our newest line of defense against bacteria. Created with BioRender.com. ... just the method needed to jumpstart the fight against antibiotic resistance. Advantages of CRISPR over...targeted two distinct beta-lactam resistance genes, again with very high specificity. This double phage system...antibiotic resistance plasmid. These bacteria also gain resistance to a lytic phage through the introduction...strategy can help us win back ground in the fight against bacterial superbugs. References 1. Citorik, Robert... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog PostPublishedMarch 10, 2015, 2:25 p.m....hybridoma lines, each producing a monoclonal antibody against a target antigen. This contrasts with polyclonal...popular monoclonal antibodies include those directed against human transcription factors such as PAX7, PAX6,... addition, we distribute monoclonal antibodies against popular fluorescent proteins (GFP and YFP) and ... high profile antigens; and 4) generating mAbs against new cancer biomarkers. We believe that repositories... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m.... fraction of readers cover the same ground again and again as they don’t save the insights they glean ...years of a project. Dissemination of the knowledge gained during this period occurs infrequently and rarely...researchers, we wondered how we could make the insights gained during this time more visible and thereby have ... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....is to keep working on telling a story again and again and again until you work out what people enjoy and...lessons you’ve learned and what knowledge you’ve gained. Show that as the story has progressed, you or ... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m.... Secondary antibodies are antibodies generated against antibody isotypes from a specific species, such...possible to get secondary antibodies generated against isotype subclasses, such as mouse IgG2 or IgG4....unbound or excess antibody. Incubate the sample again with a conjugated secondary antibody, which will...anti-rabbit?!”* *a secondary antibody generated in goat against rabbit immunoglobulins. References and Resources... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....and how do I fix it? When plotting two channels against each other, you should be able to draw perpendicular...single-color APC control, with APC and APC-CY7 plotted against each other. Since it is a single-color control,...After increasing the compensation values for APC against APC-Cy7, the populations are in more of a straight...compensation value of five was applied to APC-Cy7 against APC to correct for this bleed. A compensation value... -
Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m....Research in Sutton, located on the edge of London to gain relevant experience. I applied for this summer position... I needed more practical lab experience to later gain a full time position in a lab. I worked on the genetics...Working in industry: virology On completing my MSc, I again had to look at what type of work I would like to...entitled to a Work-Travel VISA, which means I can gain employment for up to 6 months in Australia or another... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....animal, and its B cells will churn out antibodies against the injected protein. Several weeks later, blood...protein. However, each animal will make antibodies against different protein epitopes and so variability between... protein compared to whole antibodies (Crivianu-Gaita 2016). Get more details on ScFvs in this article...//doi.org/10.1016/j.ymeth.2017.01.009 Crivianu-Gaita V, Thompson M (2016) Aptamers, antibody scFv, and... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m....was a great, positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying Nuclear...cause breast cancer and infertility or to argue against unnecessary clinic regulations, evidence-based .... I worried that, because we were speaking out against legislators and because the march had been so well-publicized...marches across the world have had an impact already, gaining additional funding and the eyes of the world. Justin... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog PostPublishedMarch 14, 2017, 2:30 p.m....bodies’ natural abilities, we’re gaining new ground in the fight against cancer in its many forms. The ...sequence of immune reactions that aid in the fight against cancer. Sipuleucel-T by the Dendreon Corporation...will more broadly activate the immune response against tumors. Some chemotherapeutic agents induce immunogenic... -
MassBioEd Career Exploration Day at Addgene
TypeBlog PostPublishedNov. 13, 2015, 3:30 p.m....many different career options in science, with Abigail Anderson commenting that “There is not just a scientist...that means it is possible to switch your field.” Abigail was also impressed with the “comfortable surroundings...surroundings” of the new Addgene office space. Thanks Abigail, we think it’s pretty great, too! Thanks to MassBioEd... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m....notice the name Vladislav Verkhusha popping up again and again, and for good reason. We all know scientists...sections of the relevant papers, and then read them again. Most of the time, the answers will be there. ... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m....Cas as a powerful and adaptive defense strategy against life-threatening viruses. Evolving to survive ...clustered repeat sequences operated as a safeguard against bacteriophages did not come to light until 2007...Cas systems provides powerful lines of defense against invading phages. Rising to the challenge - Evolution...