We narrowed to 3,637 results for: golden gate

1. pPD107 VP16-ZF43(RARR)

   Plasmid

   #138812

   Purpose

   Constitutive expression of VP16-ZF43(RARR) under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF43(RARR)
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   R15A

   Promoter

   CMV

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPD530 VP16-ZF42

   Plasmid

   #138760

   Purpose

   Constitutive expression of VP16-ZF42 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF42
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPD531 VP16-ZF54

   Plasmid

   #138762

   Purpose

   Constitutive expression of VP16-ZF54 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF54
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPD527 VP16-ZF14

   Plasmid

   #138756

   Purpose

   Constitutive expression of VP16-ZF14 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF14
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNM3746

   Plasmid

   #154109

   Purpose

   loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Inserts

   LoxP-SEC-LoxP
   FRT::rpl-28p::GFP::His-58::FRT3
   mex-5p::FLP D5::sl2::mNG::glh2 3'
   

   Use

   Cre/Lox; Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS1_4x7SKPylT_EF1_Ub-BsmBI

   Plasmid

   #162801

   Purpose

   STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-BsmBI
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPOR1CB0113

   Plasmid

   #117552

   Purpose

   Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S+5'UTR OMEGA (pICH51288)

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCALNL-BsaI-EGFP

   Plasmid

   #123136

   Purpose

   Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   EGFP, mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cBA

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGGC001

   Plasmid

   #48824

   Purpose

   Provides WUS as GreenGate coding sequence module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   WUS (WUS Mustard Weed)
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   internal BsaI recognition site removed by silent …

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBT195

    Plasmid

    #127843

    Purpose

    clone BE4max CBEs by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    BE4max without deaminase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTU2_2a

    Plasmid

    #73010

    Purpose

    Secondary module (BpiI) site in pTU2a backbone

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Secondary module + Golden Gate pTU2a BsmBI destination site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUS_A8

    Plasmid

    #99882

    Purpose

    Recipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPOR0SP0011

    Plasmid

    #117530

    Purpose

    Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-xCas9 3.7 (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLP-PrhaBAD-pcepA-himar-chlorR

    Plasmid

    #238492

    Purpose

    Landing Pad with RhaRS/PrhaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

    Depositor

    Farren Isaacs

    Article

    Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    RhaRS/PrhaBAD

    Available since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. p0AmpAB-PXdUspA

    Plasmid

    #237241

    Purpose

    Carries the X. dendrorhous UspA promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdUspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p0AmpAB-PXdGrg1

    Plasmid

    #237242

    Purpose

    Carries the X. dendrorhous Grg1 promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdGrg1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p0AmpAB-PXdADH

    Plasmid

    #237243

    Purpose

    Carries the X. dendrorhous ADH promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdADH
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p0AmpAB-PXdHP11

    Plasmid

    #237244

    Purpose

    Carries the X. dendrorhous HP11 promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdHP11
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p0AmpAB-PXdHP10

    Plasmid

    #237245

    Purpose

    Carries the X. dendrorhous HP10 promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdHP10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p0AmpAB-PXdHP9

    Plasmid

    #237246

    Purpose

    Carries the X. dendrorhous HP9 promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdHP9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge