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We narrowed to 5,185 results for: golden gate

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Showing: 2281 - 2300 of 5185 results

  3. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit
    #1000000138
    Purpose
    This toolkit allows generation of transcriptional units using GoldenBraid for integration in the S. cerevisiae genome at loci, YPRCΔ15 and YORWΔ22, to support high and stable transgene expression.
    Depositor
    Elena Caro Bernat
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (GoldenBraid)
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  4. Mobius Assembly Vector Toolkit

    Plasmid Kit
    #1000000134
    Purpose
    Mobius Assembly is a Golden Gate-based cloning framework which renders both high cloning capacity and vector toolkit simplicity.
    Depositor
    Naomi Nakayama
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    March 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. The Xenorhabdus griffiniae part library

    Plasmid Kit
    #1000000274
    Purpose
    Use this parts library for Golden Gate and Gibson assemblies to express DNA parts in the bacterium Xenorhabdus griffiniae.
    Depositor
    Richard Murray
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    Feb. 13, 2026
    Availability
    Academic Institutions and Nonprofits only

  6. FusX TALEN assembly system

    Plasmid Kit
    #1000000063
    Purpose
    Streamlined Golden Gate TALE assembly of 15–16 RVD TALEN inserts in one step that is backwards compatible with popular TALE backbones for expression in multiple systems; requires additional plasmids for use.
    Depositor
    Stephen Ekker
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Zebrafish Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. Ekker Lab TALEN kit

    Plasmid Kit
    #1000000038
    Purpose
    Complete collection for assembly of highly active 15-RVD GoldyTALENs for expression in zebrafish. The collection complements the Golden Gate TALEN and TAL Effector Kit 2.0 (#1000000024).
    Depositor
    Stephen Ekker
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Zebrafish Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. UbiGate E2 activity screen (set2)

    Plasmid Kit
    #1000000145
    Purpose
    UbiGate E2 Activity Screen (set 2) includes expression cassettes containing ubiquitin, the E1 UBA1 in combination with all Arabidopsis thaliana E2's for E3 autoubiquitination assays. See Kit #1000000144 for Set 1 components.
    Depositor
    Marco Trujillo
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  9. Cultivarium JERBOA Toolkit

    Plasmid Kit
    #1000000275
    Purpose
    Golden Gate genetic toolkit developed to help researchers identify functional transposons and establish genetic tractability in diverse non-model bacteria.
    Depositor
    Nili Ostrov
    Application
    Barcoding, Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    March 12, 2026
    Availability
    Academic Institutions and Nonprofits only

  10. 4G-Cloning Plasmid Kit

    Plasmid Kit
    #1000000252
    Purpose
    Use 4G (Golden-Gate-guided Gibson) cloning for rapid assembly of single- or multi-gene expression vectors for multi-subunit protein complexes in various expression hosts.
    Depositor
    Stephan Gruber
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only

  11. pGGG Wheat GUS marker

    Plasmid
    #165418
    Purpose
    Wheat transformation binary vector pGGG confers hygromycin resistance in wheat and contains the GUS (2x introns) reporter gene driven by OsUbi promoter
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Article
    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.
    Insert
    Os Ubiquitin Promoter::Gus (2x introns)::NosT
    Use
    Wheat transformation binary vector
    Promoter
    OS UbiP::GUS (2xint)::NosT
    Available Since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165418-plasmid-map-sequence-id-331269

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  12. pNK497

    Plasmid
    #219754
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Expression
    Plant
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219754-plasmid-map-sequence-id-458646

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  17. pBFC0984

    Plasmid
    #231992
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with 2xBsaI spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231992-plasmid-map-sequence-id-467618

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  18. Fragmid

    Plasmid Kit
    #1000000237
    Purpose
    Toolkit for rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.
    Depositor
    John Doench
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    AAV, Insect Expression, Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 2281 - 2300 of 5185 results