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pCAGGS puro HA-hUSP27X Y144C

Plasmid

#225720

Purpose

Transfection of USP27X (Y144C) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y144C (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

Y144C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X G76S

Plasmid

#225719

Purpose

Transfection of USP27X (G76S) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X G76S (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

G76S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X S404N

Plasmid

#225717

Purpose

Bacterial expression of USP27X (S404N) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X S404N (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

S404N

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X Y381H

Plasmid

#225716

Purpose

Bacterial expression of USP27X (Y381H) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y381H (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

Y381H

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X F313V

Plasmid

#225715

Purpose

Bacterial expression of USP27X (F313V) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X F313V (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

F313V

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X Y144C

Plasmid

#225713

Purpose

Bacterial expression of USP27X (Y144C) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y144C (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

Y144C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X G76S

Plasmid

#225712

Purpose

Bacterial expression of USP27X (G76S) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X G76S (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

G76S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X C87A

Plasmid

#225711

Purpose

Bacterial expression of USP27X (C87A) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X C87A (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

C87A, inactivates deubiquitylase

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCI AscI MR1 R9H res

Plasmid

#214753

Purpose

expresses human MR1 R9H resistant to CRISPR editing with a specific sgRNA in mammalian cells

Depositor

David Lewinsohn

Article

Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

Insert

MHC class I-related protein 1 (MR1 Human)

Tags

IRES eGFP

Expression

Mammalian

Mutation

silent mutations to prevent editing by CRISPR/Cas…

Promoter

CMV

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10

Plasmid

#180349

Purpose

mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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