We narrowed to 7,241 results for: 11

1. hisSUMO-ZFP57

   Plasmid

   #102716

   Purpose

   Express DNA binding domain of human Zinc finger protein 57 in E. coli

   Depositor

   Gary Stormo

   Article

   Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

   Insert

   Zinc finger protein 57 (ZNF57 Human)
   

   Use

   Tags

   hisSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET22b-T5-FB-E25TAG

   Plasmid

   #176545

   Purpose

   Expresses IgG affinity protein FB with a TAG stop codon at E25 position

   Depositor

   Han Xiao

   Article

   Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

   Insert

   IgG affinity protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   change glutamic acid 25 to amber stop codon

   Promoter

   T5

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIntegrase_10

   Plasmid

   #60581

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIntegrase_11

   Plasmid

   #60582

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_11
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG25-L-IFP-F[1] (clon 3)

   Plasmid

   #52899

   Purpose

   Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[1]
   

   Use

   Vector for homologous recombination

   Tags

   Expression

   Mutation

   Promoter

   TEF promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pAG32-L-IFP-F[2] (clon 3)

   Plasmid

   #52900

   Purpose

   Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[2]
   

   Use

   Vector for homologous recombination

   Tags

   Expression

   Mutation

   Promoter

   TEF promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

7. Rap-MESA-20-M-Gal4

   Plasmid

   #84554

   Purpose

   MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

   Depositor

   Joshua Leonard

   Article

   Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

   Insert

   Rap-MESA-FRB-20-M-Gal4
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2+-H2B-pr-mEosFP

   Plasmid

   #141101

   Purpose

   H2B fused to pr-mEosFP for chromatin visualization and primed conversion

   Depositor

   Periklis Pantazis

   Article

   Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

   Insert

   Histone 2B
   

   Use

   Tags

   pr-mEosFP

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7-agrBD-I

   Plasmid

   #53439

   Purpose

   T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrBD locus
   

   Use

   Synthetic Biology

   Tags

   V5 epitope, 6x HIS (not expressed on agrB or agrD…

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7-lac

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-C1-hEFA6B

    Plasmid

    #206413

    Purpose

    Mammalian expression of hEFA6B

    Depositor

    Hye-Won Shin, Kazuhisa Nakayama

    Article

    Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

    Insert

    EFA6B (PSD4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pReporter_2

    Plasmid

    #60563

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-NLS-ZapCmR2

    Plasmid

    #59012

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR2 genetically encoded Zn(II) sensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pNLRP11major.MYC

    Plasmid

    #131196

    Purpose

    Mammalian expression of human NLRP11 188S

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

    Insert

    NLRP11 188S (NLRP11 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    188S

    Promoter

    pCMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-C1-hRabin8 F201A

    Plasmid

    #26728

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    Rabin8 (RAB3IP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    F201A: GEF domain mutant

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

15. 645 pCS2-etv2

    Plasmid

    #49005

    Purpose

    Mammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Invitro transcription, riboprobe template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-C1-hRabin8 L196A

    Plasmid

    #26727

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    Rabin8 (RAB3IP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L196A: GEF domain mutant.

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pBlastR-5xUAS-sfGFP-GMRWhite

    Plasmid

    #165907

    Purpose

    Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FabF (KASII) pet28 N-terminal His Tag

    Plasmid

    #75017

    Purpose

    Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    FabF (fabF E. coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-nDsRedFL-miRNT

    Plasmid

    #177808

    Purpose

    A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-NT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 Tat K41A HA (P#37)

    Plasmid

    #14655

    Depositor

    Matija Peterlin

    Article

    Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

    Insert

    Tat (tat HIV)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K41A

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only