We narrowed to 32,079 results for: Eng

1. pHAGE2-TetO-GFP-mSox17

   Plasmid

   #206373

   Purpose

   Expresses EGFP fused mouse SOX17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox17 (Sox17 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF.mHES1.CMV.GFP

   Plasmid

   #17622

   Depositor

   Linzhao Cheng

   Article

   Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

   Insert

   HES1 (Hes1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 5HT2A_NB2_1

   Plasmid

   #127690

   Purpose

   Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

   Depositor

   Bryan Roth, Justin English

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   5HT2A_NB2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTREX GPR68_NB2_2

   Plasmid

   #127691

   Purpose

   Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

   Depositor

   Bryan Roth, Justin English

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   GPR68_NB2_2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSP1594

   Plasmid

   #65775

   Purpose

   Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKMV-VioD

   Plasmid

   #65350

   Purpose

   systhesis of vioD gene in the violacein pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   vioD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAID5XY

   Plasmid

   #165051

   Purpose

   Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVTHM-mSox17-3xflag

   Plasmid

   #206377

   Purpose

   Expresses 3xflag fused mouse SOX17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox17 (Sox17 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKMV-VioB

   Plasmid

   #65348

   Purpose

   systhesis of vioB gene in the violacein pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   vioB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-CMV-PDGFRa-3xFlag-EF1-nlsCopGFP

    Plasmid

    #99731

    Purpose

    overexpression of PDGFRa and CopGFP

    Depositor

    Mengsheng Qiu

    Article

    Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

    Insert

    Pdgfra (Pdgfra Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1-H-Luc_mito

    Plasmid

    #113930

    Purpose

    Plasmid encoding the mitochondrial localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc_mito
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBTK527

    Plasmid

    #110605

    Purpose

    BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pDH42(CUG/A)

    Plasmid

    #154132

    Purpose

    Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

    Insert

    engineered bacterial rRNAs operon under control of pL promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pV132-vCas9

    Plasmid

    #203223

    Purpose

    Mammalian expression of vCas9

    Depositor

    Vikash Chauhan, Robert Langer, Phil Sharp

    Article

    Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    vCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    S55R R976A K1003A T1314R

    Promoter

    CBh

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ290

    Plasmid

    #129670

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    AacCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBTK527a

    Plasmid

    #110606

    Purpose

    BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pYPQ292

    Plasmid

    #129672

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    AaCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK-Myc-Smurf1 delHECT

    Plasmid

    #13677

    Depositor

    Ying Zhang

    Article

    Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

    Insert

    Smurf1 deltaHect (SMURF1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Smurf1 without HECT domain (deleted amino acids 3…

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pYPQ141-ZmUbi-RZ-Cas12j2

    Plasmid

    #173926

    Purpose

    Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEL638

    Plasmid

    #189831

    Purpose

    The T-DNA backbone of nCas12j2 for genome editing in rice

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR; T-dna clone

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi1

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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