We narrowed to 18,031 results for: Mut

1. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-M566T

   Plasmid

   #218832

   Purpose

   Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant M566T for BioID experiments.

   Depositor

   Travis Stracker

   Insert

   TLK1 M566T (TLK1 Human)
   

   Use

   Tags

   FLAG-BirA*

   Expression

   Mammalian

   Mutation

   M566T

   Promoter

   CMV

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-Q479E

   Plasmid

   #218833

   Purpose

   Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant Q479E for BioID experiments.

   Depositor

   Travis Stracker

   Insert

   TLK1 Q479E (TLK1 Human)
   

   Use

   Tags

   FLAG-BirA*

   Expression

   Mammalian

   Mutation

   Q479E

   Promoter

   CMV

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMin1

   Plasmid

   #102959

   Purpose

   Mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions

   Depositor

   Rainer Haas

   Article

   Kahrs et al Gene. 1995 Dec 29;167(1-2):53-7.

   Type

   Empty backbone

   Use

   Mini-transposon system suitable for sequencing, s…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK501

   Plasmid

   #72246

   Purpose

   Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gln206 to Arg and changes tryptophan-151 to pheny…

   Promoter

   T7 and endogenous

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK600

   Plasmid

   #71605

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, histidine…

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHoss1

   Plasmid

   #63158

   Purpose

   Suicide plasmid for efficient gene mutation in Gram-positive bacteria

   Depositor

   Attila Karsi

   Article

   Abdelhamed et al Plasmid. 2015 May 31;81:1-8. doi: 10.101

   Insert

   anti-secY-Pxyl/tetO-tetR
   

   Use

   Suicide plasmid for gram-positive bacteria

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1 Bach1 S990A

   Plasmid

   #73643

   Purpose

   Mammalian expression of Bach1 S990A mutant (BRCA binding mutant).

   Depositor

   Sharon Cantor

   Insert

   Bach1 (BACH1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   S990A (BRCA binding mutant)

   Promoter

   CMV

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pTETSIX

   Plasmid

   #74104

   Purpose

   mCherry-encoding vector for markerless deletion mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria

   Depositor

   Hans-Martin Fischer

   Article

   Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

   Insert

   tetA(C)
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   C-to-T transition at pos. no. 1049 of tetA(C) lea…

   Promoter

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCZ886

   Plasmid

   #73584

   Purpose

   Pmex-5-HIS-72-miniSOG-3'UTR(tbb-2) for optogenetic mutagenesis

   Depositor

   Yishi Jin

   Article

   Noma et al Nat Commun. 2015 Dec 3;6:8868. doi: 10.1

   Insert

   HIS-72 (his-72 Nematode)
   

   Use

   For mossci insertion

   Tags

   miniSOG

   Expression

   Worm

   Mutation

   Promoter

   Pmex-5

   Available Since

   March 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTGFP-T7-Hha10comp

    Plasmid

    #65428

    Purpose

    Competitor vector for transcriptional mutagenesis assay

    Depositor

    Yinsheng Wang

    Article

    You et al Nat Protoc. 2015 Sep;10(9):1389-406. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. G1S-HA-pCB6

    Plasmid

    #74373

    Purpose

    Mammalian expression of a G1S mutant HA from the X:31 strain of influenza virus

    Depositor

    Judith White

    Article

    Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

    Insert

    HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G1S

    Promoter

    CMV

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

12. G1V-HA-pCB6

    Plasmid

    #74372

    Purpose

    Mammalian expression of a G1V mutant HA from the X:31 strain of influenza virus

    Depositor

    Judith White

    Article

    Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

    Insert

    HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G1V

    Promoter

    CMV

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

13. N-SF-TAP-DEST-TLK2-D592V

    Plasmid

    #218835

    Purpose

    Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).

    Depositor

    Travis Stracker

    Insert

    TLK2 Q592V (kinase dead) (TLK2 Human)
    

    Use

    Tags

    FLAG-STREP

    Expression

    Mammalian

    Mutation

    Q592V

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKP1627

    Plasmid

    #167648

    Purpose

    Express FepA G54C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.

    Depositor

    Kathleen Postle

    Article

    Postle et al bioRxiv 2021.12.08.471779

    Insert

    fepA G54C, delta12-16 (TonB box)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    fepA with G54C, delta12-16 (TonB box) mutations (…

    Promoter

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCh-cpFRB1

    Plasmid

    #155389

    Purpose

    Express cpFRB1 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    cpFRB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCh-cpFRB2

    Plasmid

    #159645

    Purpose

    Express cpFRB2 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    cpFRB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ad-EEF1D-S133E

    Plasmid

    #131577

    Purpose

    Expresses EEF1D with S133E mutation in mammalian cells

    Depositor

    Haiyan Xu

    Insert

    Eukaryotic translation elongation factor 1 delta (EEF1D Human)
    

    Use

    Adenoviral

    Tags

    No

    Expression

    Mutation

    changed Serine 133 to Glutamic acid

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK500

    Plasmid

    #72245

    Purpose

    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    T. J. Kappock

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and endogenous

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hs.NRAS G12D

    Plasmid

    #83176

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12D

    Promoter

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30b-7+-SrtA

    Plasmid

    #105602

    Purpose

    expressed 7+ sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7+ mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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