We narrowed to 6,138 results for: WUS;

1. pAAV-CAG-tTA

   Plasmid

   #99118

   Purpose

   An AAV genome that ubiquitously expresses tTA from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV2

   Insert

   tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   CAG

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR

   Plasmid

   #176311

   Purpose

   Expresses NLS-pMag-CreC60 with tagBFP marker

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1alpha

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3 Flag MKK4

   Plasmid

   #14615

   Depositor

   Roger Davis

   Article

   Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

   Insert

   MKK4 (MAP2K4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a_Pro-IL-1α

   Plasmid

   #214341

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL1A (IL1A Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. (133) pcDNA3.1-Nup62-Flag-EPEA

   Plasmid

   #160744

   Purpose

   Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   Nup62-Flag-EPEA
   

   Use

   Tags

   EPEA and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

   Plasmid

   #190162

   Purpose

   Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

   Insert

   mcherry_(3'UTR, No Actin)_24Broccoli
   

   Use

   Tags

   24×Broccoli

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac_human-IL-18

   Plasmid

   #214338

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LifeAct-GFP-cytoB5RR

   Plasmid

   #182577

   Purpose

   Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   LifeAct-GFP-cytoB5RR
   

   Use

   Tags

   LifeAct and cytoB5RR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMB-SaCas9

   Plasmid

   #105998

   Purpose

   SaCas9 expression vector

   Depositor

   Mike McManus

   Article

   Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

   Insert

   Cas9 nuclease
   

   Use

   CRISPR and Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   short EF1alpha

   Available since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-mouse vinculin full length (889)

    Plasmid

    #67935

    Purpose

    Mammalian expression of mouse vinculin with GFP fusion

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    Vinculin (Vcl Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L159P in vinculin

    Promoter

    CMV

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-CMV-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204339

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-FLAG-BMAL1

    Plasmid

    #186829

    Purpose

    Expresses FLAG-BMAL1 in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-hSyn-Galpha-i1 ONE-GO

    Plasmid

    #204333

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-i1 ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pST1374-NLS-flag-linker-Cas9

    Plasmid

    #44758

    Depositor

    Xingxu Huang

    Article

    Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

    Insert

    Human codon optimized Cas9
    

    Use

    CRISPR

    Tags

    32 amino acid linker, Flag, and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDA019

    Plasmid

    #131452

    Purpose

    control expression vector

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 NLRP1

    Plasmid

    #166823

    Purpose

    Gateway pDONR vector containing NLRP1 (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-hSyn-Galpha-oA ONE-GO

    Plasmid

    #204335

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-oA ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTK2_007

    Plasmid

    #123702

    Purpose

    Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pEF1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDual-IN-SMN1

    Plasmid

    #215721

    Purpose

    dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN1 EXON 7 (SMN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge