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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,493 results for: D10

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Showing: 2321 - 2340 of 2493 results

  1. pSCBE3-HF1

    Plasmid
    #218156
    Purpose
    This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218156-plasmid-map-sequence-id-434901

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  2. pSCBE3-Hypa

    Plasmid
    #218157
    Purpose
    This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N692A…
    Promoter
    rpsL promoter
    Available since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    218157-plasmid-map-sequence-id-433172

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  3. pFBD-BirA_ATG16L1:303-607

    Plasmid
    #210942
    Purpose
    Insect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Publication
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Insert
    ATG16L1:G303-Y607 (ATG16L1 Human)
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 4, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210942-plasmid-map-sequence-id-424570

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  8. PB-LAMP1-AlfaTag-APEX2

    Plasmid
    #253247
    Purpose
    Express LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. LAMP1 is also fused to AlfaTag for IF or WB detection.
    Depositor
    May Malicdan
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Piggybac transposon
    Tags
    AlfaTag-APEX2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    253247-plasmid-map-sequence-id-505397

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  9. PB-LAMP1-3xFLAG-APEX2

    Plasmid
    #253249
    Purpose
    Express LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. LAMP1 is also fused to 3xFlag for IF or WB detection.
    Depositor
    May Malicdan
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Piggybac transposon
    Tags
    3xFALG-APEX2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    253249-plasmid-map-sequence-id-503910

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  14. pRhaB-8xHis-ABE8e-eVRQR (LLH551)

    Plasmid
    #242658
    Purpose
    pRhaB promoter expression plasmid for ABE8e-eVRQR with an N-terminal His8-tag
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pRhaB-8xHis-BPNLS-TadA8e-SpCas9(D10A)-eVRQR-BPNLS
    Use
    CRISPR
    Tags
    8x-His
    Expression
    Bacterial
    Mutation
    eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…
    Promoter
    RhaB
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    242658-plasmid-map-sequence-id-491546

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Showing: 2321 - 2340 of 2493 results