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MAP3K7CL_pCSdest

Plasmid

#53808

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MAP3K7CL (C21orf7 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pECE M2-SNX17 S437A

Plasmid

#69812

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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CHAF1B_pCSdest

Plasmid

#53862

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CHAF1B (CHAF1B Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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xfz7N/pCS2+

Plasmid

#16325

Depositor

Peter Klein

Article

Deardorff et al Development. 1998 Jul . 125(14):2687-700

Insert

frizzled-7 (fzd7.L Frog)

Use

Vertebrate expression

Mutation

Amino terminal end

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
HOXB13 (human) Myc-tag-Bluescript

Plasmid

#8684

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Tags

Myc

Expression

Bacterial

Mutation

Myc tag on N-terminal

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCSIIpuro-EGF-ScNeo

Plasmid

#209893

Purpose

To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

EGF-ScNeo (EGF Human)

Use

Lentiviral

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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PB-NLS-ZapCV2-ALA

Plasmid

#203665

Purpose

Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells

Depositor

Amy Palmer

Article

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2-Ala zinc dead sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

Cys and His in Zap1 is mutated with Ala

Promoter

CMV promoter

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

Plasmid

#180011

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-mAV

Plasmid

#129472

Purpose

Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)

Depositor

Elizabeth Luna

Article

Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-

Insert

Supervillin, isoform 2 (Svil Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe(puro) mouse Egr4

Plasmid

#108105

Purpose

Retroviral vector for expression of mouse Egr4

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr4 (Egr4 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pSirenRetroQ(puro)-shEgr4

Plasmid

#108117

Purpose

pSIREN-RetroQ vector containing shRNA sequence to Egr4

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

shRNA to Egr4 (Egr4 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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p3-HPRT1-S104R-PdTK-mCh

Plasmid

#107276

Purpose

HPRT1 c.312C>A Munich donor vector with unilateral microhomology

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Inserts

HPRT genomic DNA (HPRT1 Human)
puro-deltaTK; CAG::mCherry

Mutation

c.306G>T, c.312C>A

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRVdG-mCherry-N-P-M-nucBFP-EGFP-L

Plasmid

#52484

Purpose

Expresses mCherry, nuclear mTagBFP and EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mCherry
nuclear mTagBFP
EGFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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CS2-GFP-Ben

Plasmid

#47966

Purpose

Expression of fly Ben

Depositor

Laura Lee

Article

Merkle et al Development. 2009 Feb;136(3):449-59. doi

Insert

Bendless (ben Fly)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pAG252

Plasmid

#33206

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

MAP4K4 3'UTR (MAP4K4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40 early enhancer/promoter

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos DD Y345A

Plasmid

#8971

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362D, S374D, Y345A

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos DD F343A

Plasmid

#8972

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362D, S374D, F343A

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
Lenti-CMV-PE2-IRES-ZeoR

Plasmid

#207352

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

Prime editor 2 (PE2) enzyme

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Cdx2-3xT7

Plasmid

#200891

Purpose

Inducible lentiviral expression of Cdx2

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Cdx2 (Cdx2 Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 WT

Plasmid

#182046

Purpose

expression of Sox2

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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