We narrowed to 5,844 results for: pCas

1. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176238

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG1.1-Armc12 delta ARM/FLAG

   Plasmid

   #180496

   Purpose

   Expression vector of ARM domain deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

   Insert

   armadillo repeat containing 12 (Armc12 Mouse)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   ARM domain of Armc12 is deleted

   Promoter

   CAG promoter

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9(1.1)-ABL1-gRNA1-exon4

   Plasmid

   #163322

   Purpose

   eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ABL1 gRNA 1_Exon4
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

4. eSpCas9(1.1)-ABL1-gRNA2-exon4

   Plasmid

   #163323

   Purpose

   eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ABL1 gRNA 2_Exon4
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

5. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

   Plasmid

   #165083

   Purpose

   gRNA 2 of pair B for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

   Plasmid

   #165085

   Purpose

   gRNA 1 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

   Plasmid

   #165086

   Purpose

   gRNA 2 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-mcherry-WIPI2dH85E-cs-TEV-STREP

   Plasmid

   #171838

   Purpose

   Mammalian expression of human WIPI2d H85E with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   Strep and mCherry

   Expression

   Mammalian

   Mutation

   H85E

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-mcherry-WIPI2dK88E-cs-TEV-STREP

   Plasmid

   #171839

   Purpose

   Mammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   Strep and mCherry

   Expression

   Mammalian

   Mutation

   K88E

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-mcherry-WIPI2dI92E-cs-TEV-STREP

    Plasmid

    #171840

    Purpose

    Mammalian expression of human WIPI2d I92E with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    I92E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-mcherry-WIPI2dC93E-cs-TEV-STREP

    Plasmid

    #171841

    Purpose

    Mammalian expression of human WIPI2d C93E with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    C93E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP

    Plasmid

    #171842

    Purpose

    Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    R108E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-mcherry-WIPI2dR125E-cs-TEV-STREP

    Plasmid

    #171843

    Purpose

    Mammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    R125E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP

    Plasmid

    #171844

    Purpose

    Mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep and mCherry

    Expression

    Mammalian

    Mutation

    K128E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-OSF-PP-squirrel-monkey-CHMP3

    Plasmid

    #154182

    Purpose

    expresses squirrel monkey CHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3
    

    Tags

    FLAG, Strep-Tag II, and preScission site

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-OSF-PP-squirrel-monkey-retroCHMP3

    Plasmid

    #154184

    Purpose

    expresses squirrel monkey retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Tags

    FLAG, Strep-Tag II, and preScission site

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCaggs-NLS-PAmCherry1-GSS-EGFP

    Plasmid

    #158000

    Purpose

    Expression of nucleus localized PAmCherry1 and EGFP fusion protein

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    NLS-PAmCherry1-GSS-EGFP
    

    Expression

    Mammalian

    Promoter

    Actin promoter with CMV enhancer

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAA)

    Plasmid

    #137860

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAAA)

    Plasmid

    #137861

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAAA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSA)

    Plasmid

    #137862

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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