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Showing: 5901 - 5950 of 6949 results

  3. Adeno BN

    Plasmid
    #101823
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1
    Depositor
    Andrea Ventura
    Article
    Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10
    Insert
    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)_CBh_FLAG-Cas9 (Bcan, Ntrk1 Mouse)
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  12. AAV-GFP/Cre

    Plasmid
    #49056
    Purpose
    Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.
    Depositor
    Fred Gage
    Article
    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99
    Insert
    Cre
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    EGFP, Myc, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    49056-plasmid-map-sequence-id-182646

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  15. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Christopher Johnson, Gregg Beckham
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112204-plasmid-map-sequence-id-215853

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  17. SARS-CoV2 Nucleocapsid

    Plasmid
    #153201
    Purpose
    Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells
    Depositor
    Peter Klein
    Article
    Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11
    Insert
    Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Use
    Tags
    Myc tag (5X)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 15, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153201-plasmid-map-sequence-id-305776

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  20. pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE

    Plasmid
    #166970
    Purpose
    Cre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.
    Depositor
    Matthew Banghart
    Article
    Liu et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    Homo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
    Use
    AAV and Cre/Lox
    Tags
    FLAG, T2A, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    hsyn
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166970-plasmid-map-sequence-id-346867

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  28. eGFP-proα2(I)-G610C

    Plasmid
    #119827
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119827-plasmid-map-sequence-id-233848

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  32. PLVX_SPTA1_IGF2BP1_IRES_PURO

    Plasmid
    #100016
    Purpose
    To over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.
    Depositor
    Jeffery Miller
    Article
    de Vasconcellos et al Proc Natl Acad Sci U S A. 2017 Jul 11;11
    Insert
    Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SPTA1 erythroid specific promoter
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100016-plasmid-map-sequence-id-194507

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  35. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid
    #220611
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220611-plasmid-map-sequence-id-441218

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  36. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162675-plasmid-map-sequence-id-321470

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  37. pETDuet-TFIIF

    Plasmid
    #171082
    Purpose
    Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    RAP30 (GTF2F2 Human)
    RAP74 (GTF2F1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171082-plasmid-map-sequence-id-335551

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  38. pCAGGS-PHFF-sh-hBVRA

    Plasmid
    #100285
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    Use
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100285-plasmid-map-sequence-id-194439

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  40. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid
    #204215
    Purpose
    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.
    Depositor
    Guei-Sheung Liu
    Article
    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121
    Insert
    Cas13bt3 and U6.SapI sgRNA cloning site
    Use
    AAV and CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Codon optimisation by GenScript tool
    Promoter
    CMV/U6
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    204215-plasmid-map-sequence-id-455913

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  46. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220608
    Purpose
    Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220608-plasmid-map-sequence-id-441203

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  47. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220612
    Purpose
    Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220612-plasmid-map-sequence-id-441117

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  50. mCherry-CRY2-INPP5E

    Plasmid
    #79561
    Purpose
    expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 5901 - 5950 of 6949 results