We narrowed to 3,461 results for: mpo

1. pNK1508

   Plasmid

   #219750

   Purpose

   pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - npgA - tAOX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sec61β-Flag-APEX2-SOPP3

   Plasmid

   #229072

   Purpose

   Expresse APEX2-SOPP3 in ER in mammalian cell

   Depositor

   Weijun Pan

   Article

   Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

   Insert

   Express APEX2-SOPP3 in the ER (APEX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

   Plasmid

   #170273

   Purpose

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Comments

   Promoter

   CMVd1

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR EGFR-SLP76-TagBFP

   Plasmid

   #188630

   Purpose

   Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   EGFR (EGFR Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   SLP76-TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SHMT2-RES

   Plasmid

   #72880

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

   Insert

   SHMT2 (SHMT2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   silent mutations in site corresponding to shSHMT2…

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Lenti-pHSE::Cre-mRuby3

   Plasmid

   #164136

   Purpose

   Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   HSE promoter-Cre-T2A-mRuby3
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HSE synthetic promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. H2B-GFP-Aurora B WT

   Plasmid

   #184042

   Purpose

   Expresses H2B-GFP fused to Aurora B WT

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   AURKB (AURKB Human)
   

   Use

   Tags

   EGFP and H2B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX020

   Plasmid

   #167146

   Purpose

   MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   fungal caffeoylpiruvate hydrolase, nnCPH
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/NDUFV2/myc-His

   Plasmid

   #127491

   Purpose

   Expresses myc-tagged NDUFV2 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFV2 (NDUFV2 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXL002-ishRNA-beta-catenin-1

    Plasmid

    #36297

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    shRNA of beta-catenin-1 (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    IRES-RFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-H1

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

11. FKBP-beta2(616-951)-GFP

    Plasmid

    #100726

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform

    Promoter

    CMV

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-CMV-XRCC1-EGFP-Hygro

    Plasmid

    #176062

    Purpose

    EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    X-ray repair cross complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. BcLOV4 fusion tools screening plasmid #1

    Plasmid

    #174511

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

    Plasmid

    #188634

    Purpose

    HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Human, Synthetic)
    

    Use

    CRISPR

    Tags

    CD3ε-mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. RFP-(SUMO)10-(SIM)6

    Plasmid

    #122026

    Purpose

    Mammalian expression plasmid for multivalent scaffold of (SUMO)10-(SIM)6 biomolecular condensates to recruit SIM-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 copies of human SUMO3 and 6 repeats of human SUMO interactive motif (SIM) from PIASx separated by (GGS)4 linker (SUMO3, PIAS2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pN094

    Plasmid

    #167155

    Purpose

    MoClo-compatible Level1 (position 6) vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-npgA-ocsT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MBP-CHC(1-1074)-His6

    Plasmid

    #100748

    Purpose

    Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CLTC (CLTC Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Residues 1-1074

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX4T1_GST-PLproCS-MBP

    Plasmid

    #169195

    Purpose

    To express a PLpro substrate (GST-PLproCS-MBP) in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    GST-PLproCS-MBP
    

    Use

    Tags

    GST-MBP fusion (PLpro-cleavable) and His6 (intern…

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXL003-ishRNA-beta-catenin-2

    Plasmid

    #36299

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    shRNA of beta-catenin-2 (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    IRES-RFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-H1

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

20. RFP-(SUMO)6-(SIM)10

    Plasmid

    #122027

    Purpose

    Mammalian expression plasmid for multivalent scaffold of (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    6 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2, SUMO3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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