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We narrowed to 760 results for: mpo

Showing: 1 - 20 of 760 results

  1. Components of CRISPR/Cas9

    Type
    Blog Post
    ...describe the functions of the various CRISPR/Cas9 components. Please note that while this post is intended...intended to provide a general overview of CRISPR components, new Cas9 variants are being discovered all the ... thereof. This individual protein has all the components necessary to: 1. Bind to a guide RNA The guide...   Now that you know about all the different components of CRISPR, It's time to start thinking about ...

  3. Important Considerations When Using AAVs

    Type
    Blog Post
    ...and using AAVs have in common? The three most important items when looking for a house and choosing an...this mantra to AAV selection, there are three important factors related to location. Location (1): What...

  4. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...The second Boston Symposium on the Future of Research will be held from 22-24 October. This blog has ...contributed by guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar...discussions to be held at the Future of Research Symposium Boston 2015 – Academic Data and the Labor Market...Market. For more information on the symposium as a whole and for registration please go to http://futureofresearch.org...futureofresearch.org/boston/. The Future of Research Symposium A year ago, trainees from around Boston met to...research sector at our first Future of Research symposium. The talks and workshops held at this event added... the four workshops at the Future of Research Symposium 2014, young scientists are acutely aware of these...

  5. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...but, in today’s post, we’ll discuss some of the important considerations you should think about when developing...ICSS can now be done with neuronal cell-type and temporal precision. In ICSS, a test chamber is used in ...interested in studying a brain region that may be important in memory, an appropriate behavioral test might...more precisely characterized with spatial (CA1), temporal (real-time inhibition), and behavioral (remote...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow...

  6. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...Policy Group to organize a Minisymposium on Reproducibility. The minisymposium focused on solutions to reproducibility...use computer based analysis techniques, an important component of reproducibility is making sure that others... both in preparation for my talk and at the minisymposium. You can find a variety of additional resources...findings can be predictably applied to future work. Importantly, no one should expect that every study should...scientific experts discussed by Pamela Hines Minisymposium on Reproducibility Booklet - Including descriptions...

  7. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... the symposium and live tweeted throughout the event (look for a ton of tweets from the Symposium under...the topics discussed during the symposium will prove incredibly important to the stability of the biological...takeaways I got from the symposium. The goal of the Future of Research Symposium On the FOR website, the...inclusion The final and possibly the most important panel of the symposium was on diversity in academic research...sponsored the Future of Research (FOR) Boston Symposium 2015. I personally participated as a member of...main question they wanted to focus on during the symposium was “How many graduate students/post-docs?” You... clear throughout the various sessions of the symposium. Structural problems in academic research and ...

  10. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...-hyg will be used to create gene deletions of important unknown regulatory regions in Pseudomonas and ...functions in biofilm formation. The quest for plant compounds to treat complications of diabetes Gloria Otito...Her goal is to identify and isolate pure plant compounds with antidiabetic properties that can ameliorate...expression systems here) and the activity of the plant compounds on these proteins will be tested. Investigating...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...research related to Alzheimer's Disease, ALS, Frontotemporal Dementia, Huntington's Disease, Parkinson's... (AD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal dementia (FTD), Huntington's Disease (HD), ... this page. Disease Alzheimer's Disease ALS Frontotemporal dementia Huntington's Disease Parkinson's Disease...'s Alexander Stark 71083 pFOSGE_CUS1A07 GRN Frontotemporal dementia (FTD) Alexander Stark 71433 pmGFP10C...)_Paragranulin+linker 1 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176917 pcDNA3.1..._Paragranulin-no linker GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176918 pcDNA3.1...pcDNA3.1(+)_Granulin 1+linker2 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176919 pcDNA3.1...

  12. Plan Your Experiment

    Type
    Collection
    ...you are ready to start designing the specific components (Cas enzyme and guide RNA) for your experiment...genetic manipulations require different CRISPR components. Selecting the genetic manipulation you plan ...plan to perform will help you narrow down which components you’ll need for a given experiment. In the table... will cover strategies for designing the gRNA component for each category. Genetic Manipulation Application...testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference : Target...Predicted On-Target and Off-Target Activity Another important factor to keep in mind when designing your gRNA...In addition to off-target activity, it is also important to consider factors that maximize cleavage of ...

  13. TALEN Guide

    Type
    Collection
    ... Similar in principle to the ZF nuclease, the components of the array recognize individual nucleotides...Xanthomonas. The central TAL targeting domain is composed of 33-35 amino acid repeats. These repeats only...high. Since ZF targets are confined to sequences composed of triplets with corresponding zinc fingers, potential...every 35bp. Researchers are still determining the importance of context for each TAL effector within an array...TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The kit can be used...plasmid toolbox. Addgene has rapidly become an important resource for this new technology, and we hope ...

  14. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...recruiting the transposase (comprised of multiple components) to target locations in the genome defined by...V-K. Type I systems use a type I Cas effector, composed of several separate Cas enzymes that form a complex...for a ntiviral de fense), and four transposase components (TnsA, TnsB, TnsC, and TniQ). Some type I CASTs...applications where orientation of the insert is important. However, type I-F CASTs favor insertion in the...V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, and TniQ). Unlike type I, type V-K...

  15. CRISPR Guide

    Type
    Collection
    ...approach. Engineered CRISPR systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated...Cas enzyme). The gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding...mechanisms of CRISPR and some of the ways the CRISPR components are leveraged to achieve specific goals, such...Cas9 fused to proteins that modulate HDR or NHEJ components, like miCas9 , Cas9-DN1S , or others, can increase...the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit experimentally...change. Modern base editors include several fused components that promote the intended repair pathway or inhibit...V-K. Type I systems use a type I Cas effector, composed of several separate Cas enzymes that form a complex...

  16. Antibody Guide

    Type
    Collection
    ...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a...antibodies only bind a single epitope, so it is important to choose a monoclonal antibody that will recognize...conjugating and signaling molecules, as some buffer components can interfere with the conjugate or conjugation...with conjugating reactions are possible. It is important to research your reaction and buffer before conjugating... the basic steps needed for each application, important controls, and any special considerations to think... assay, selection of the fluorophores used is important. It is best to inversely pair the brightness of...no antibodies are available, change the least important factor (for instance, looking for a different ...

  17. Immunology Research Plasmids and Resources

    Type
    Collection
    ... Synonyms C3 complement component 3 ARMD9, ASP, CPAMD1 C5 complement component 5 CPAMD4, FLJ17816, FLJ17822...1, SF20 C3 complement component 3 ARMD9, ASP, CPAMD1 C3AR1 complement component 3a receptor 1 AZ3B, C3AR...C5 complement component 5 CPAMD4, FLJ17816, FLJ17822, MGC142298 C5AR1 complement component 5a receptor ...Antibodies produced by B cells activate immune components that also lead to the destruction of pathogens...grouped into tables based on the Gene Lists from Immport . Click the links below to go to the gene table...TRRP4AP, TRUSS UBR1 ubiquitin protein ligase E3 component n-recognin 1 JBS, MGC142065, MGC142067 UBXN1 UBX...IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 CR2 complement component (3d/Epstein Barr virus) receptor 2 C3DR, CD21,...

  18. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Addgene assists importation by including thorough shipping documents and following import procedure, it ...it is the responsibility of the importer to pay for any additional customs fees or taxes, and to complete... that might be required by your country for the import of biological research materials. Please be aware...

  19. Tetracycline Inducible Expression

    Type
    Collection
    ...elements like the H1 RNA promoter. And, most importantly, the TetR protein has been modified to provide...Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how to use them, or skip ahead...identify the amino acid residues of TetR that were important for tetracycline-dependent repression. Mutating... Systems Subsequent efforts have improved the components of these systems for better efficiency, sensitivity... transfected or transduced cells express both components in the same relative ratio. Different generations...

  20. Cancer Research Plasmids and Resources

    Type
    Collection
    ...extremely important for classification and treatment. On each pathway page, click on a component to find...involved in cancer development. These pathways are important to normal cell signaling and physiology, and, ...
Showing: 1 - 20 of 760 results