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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 53 results for: mpo

Showing: 1 - 20 of 53 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...research related to Alzheimer's Disease, ALS, Frontotemporal Dementia, Huntington's Disease, Parkinson's... (AD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal dementia (FTD), Huntington's Disease (HD), ... this page. Disease Alzheimer's Disease ALS Frontotemporal dementia Huntington's Disease Parkinson's Disease...'s Alexander Stark 71083 pFOSGE_CUS1A07 GRN Frontotemporal dementia (FTD) Alexander Stark 71433 pmGFP10C...)_Paragranulin+linker 1 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176917 pcDNA3.1..._Paragranulin-no linker GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176918 pcDNA3.1...pcDNA3.1(+)_Granulin 1+linker2 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176919 pcDNA3.1...

  2. Plan Your Experiment

    Type
    Collection
    ...you are ready to start designing the specific components (Cas enzyme and guide RNA) for your experiment...genetic manipulations require different CRISPR components. Selecting the genetic manipulation you plan ...plan to perform will help you narrow down which components you’ll need for a given experiment. In the table... will cover strategies for designing the gRNA component for each category. Genetic Manipulation Application...testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference : Target...Predicted On-Target and Off-Target Activity Another important factor to keep in mind when designing your gRNA...In addition to off-target activity, it is also important to consider factors that maximize cleavage of ...

  3. TALEN Guide

    Type
    Collection
    ... Similar in principle to the ZF nuclease, the components of the array recognize individual nucleotides...Xanthomonas. The central TAL targeting domain is composed of 33-35 amino acid repeats. These repeats only...high. Since ZF targets are confined to sequences composed of triplets with corresponding zinc fingers, potential...every 35bp. Researchers are still determining the importance of context for each TAL effector within an array...TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The kit can be used...plasmid toolbox. Addgene has rapidly become an important resource for this new technology, and we hope ...

  4. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...recruiting the transposase (comprised of multiple components) to target locations in the genome defined by...V-K. Type I systems use a type I Cas effector, composed of several separate Cas enzymes that form a complex...for a ntiviral de fense), and four transposase components (TnsA, TnsB, TnsC, and TniQ). Some type I CASTs...applications where orientation of the insert is important. However, type I-F CASTs favor insertion in the...V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, and TniQ). Unlike type I, type V-K...

  5. CRISPR Guide

    Type
    Collection
    ...approach. Engineered CRISPR systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated...Cas enzyme). The gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding...mechanisms of CRISPR and some of the ways the CRISPR components are leveraged to achieve specific goals, such...Cas9 fused to proteins that modulate HDR or NHEJ components, like miCas9 , Cas9-DN1S , or others, can increase...the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit experimentally...change. Modern base editors include several fused components that promote the intended repair pathway or inhibit...V-K. Type I systems use a type I Cas effector, composed of several separate Cas enzymes that form a complex...

  6. Antibody Guide

    Type
    Collection
    ...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a...antibodies only bind a single epitope, so it is important to choose a monoclonal antibody that will recognize...conjugating and signaling molecules, as some buffer components can interfere with the conjugate or conjugation...with conjugating reactions are possible. It is important to research your reaction and buffer before conjugating... the basic steps needed for each application, important controls, and any special considerations to think... assay, selection of the fluorophores used is important. It is best to inversely pair the brightness of...no antibodies are available, change the least important factor (for instance, looking for a different ...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ... Synonyms C3 complement component 3 ARMD9, ASP, CPAMD1 C5 complement component 5 CPAMD4, FLJ17816, FLJ17822...1, SF20 C3 complement component 3 ARMD9, ASP, CPAMD1 C3AR1 complement component 3a receptor 1 AZ3B, C3AR...C5 complement component 5 CPAMD4, FLJ17816, FLJ17822, MGC142298 C5AR1 complement component 5a receptor ...Antibodies produced by B cells activate immune components that also lead to the destruction of pathogens...grouped into tables based on the Gene Lists from Immport . Click the links below to go to the gene table...TRRP4AP, TRUSS UBR1 ubiquitin protein ligase E3 component n-recognin 1 JBS, MGC142065, MGC142067 UBXN1 UBX...IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 CR2 complement component (3d/Epstein Barr virus) receptor 2 C3DR, CD21,...

  8. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Addgene assists importation by including thorough shipping documents and following import procedure, it ...it is the responsibility of the importer to pay for any additional customs fees or taxes, and to complete... that might be required by your country for the import of biological research materials. Please be aware...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...elements like the H1 RNA promoter. And, most importantly, the TetR protein has been modified to provide...Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how to use them, or skip ahead...identify the amino acid residues of TetR that were important for tetracycline-dependent repression. Mutating... Systems Subsequent efforts have improved the components of these systems for better efficiency, sensitivity... transfected or transduced cells express both components in the same relative ratio. Different generations...

  10. Cancer Research Plasmids and Resources

    Type
    Collection
    ...extremely important for classification and treatment. On each pathway page, click on a component to find...involved in cancer development. These pathways are important to normal cell signaling and physiology, and, ...

  11. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...protein is often tied to its function, so it's important to determine where your protein of interest resides...2XG4S-mCer3 Mitochondria COX IV-derived (1-22 aa) import sequence and COX VIII signal peptide dL5 FAP; mCerulean3... and adhesions between animal cells. They are important for holding cell structures together and reducing...table below to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene/Signal Fluorescent...

  12. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... mEGFP Fibrillarin Nucleolus (dense fibrillar component) 87428 MYH10-mEGFP AICSDP-24 mEGFP Non-muscle ...AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component) 109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin-43...69 mTagRFP-T Nucleophosmin Nucleolus (granular component) 133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar...

  13. Promega Plasmid Collection

    Type
    Collection
    ... of a chemical compound and its target protein. The apparent affinity of test compounds is measured by...is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa) subunit and a small...

  14. p53 Pathway

    Type
    Collection
    ... of p53’s pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...Phosphatase and tensin homolog PUMA BCL2 binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...biomolecule while largely preserving spatial and temporal cellular processes. Note that many of the articles...RGB-color intensiometric indicators visualize spatiotemporal dynamics of ATP in single cells. Angew Chem...or Cre-dependent) A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics...HT3.0 Dual-color GRAB sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566...lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1...

  16. Lentivirus Plasmids

    Type
    Collection
    ...safety feature of lentiviral vectors is that the components necessary to produce an infectious viral particle...Vector Guide for more information about lentiviral components, generations, viral production, and viral safety...transfer plasmids along with additional packaging components. Didier Trono 12260 psPAX2 2nd Contains gag ,...

  17. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...University changed the way researchers can explore important questions surrounding serine phosphorylation by... of the popular SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus...#188531 ; #188532 ; #188533 ; #188534 ; #188535 ). Importantly, phosphorylation positional isomers are allocated...

  18. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...collection differ based on some important criteria, and it is important to know which library best...best suits your particular experiment. Some important variables include: Type of genetic modification - ...

  19. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...pyogenes Neo Docampo tdTomato/pTREX-b 68709 Other/Trypanosoma cruzi none S. pyogenes Blast Docampo pDonor_hU6... and plasmids that provide Cas9 variants. All components are then assembled into a single T-DNA binary...

  20. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...attributed in part to the difficulties in getting all components of the Cascade complex to express equally. To...though many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism...
Showing: 1 - 20 of 53 results