...describe the functions of the various CRISPR/Cas9 components. Please note that while this post is intended...intended to provide a general overview of CRISPR components, new Cas9 variants are being discovered all the ... thereof. This individual protein has all the components necessary to: 1. Bind to a guide RNA The guide...   Now that you know about all the different components of CRISPR, It's time to start thinking about ...

...The second Boston Symposium on the Future of Research will be held from 22-24 October. This blog has ...contributed by guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar...discussions to be held at the Future of Research Symposium Boston 2015 – Academic Data and the Labor Market...Market. For more information on the symposium as a whole and for registration please go to http://futureofresearch.org...futureofresearch.org/boston/. The Future of Research Symposium A year ago, trainees from around Boston met to...research sector at our first Future of Research symposium. The talks and workshops held at this event added... the four workshops at the Future of Research Symposium 2014, young scientists are acutely aware of these...

...but, in today’s post, we’ll discuss some of the important considerations you should think about when developing...ICSS can now be done with neuronal cell-type and temporal precision. In ICSS, a test chamber is used in ...interested in studying a brain region that may be important in memory, an appropriate behavioral test might...more precisely characterized with spatial (CA1), temporal (real-time inhibition), and behavioral (remote...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow...

...and using AAVs have in common? The three most important items when looking for a house and choosing an...this mantra to AAV selection, there are three important factors related to location. Location (1): What...

... the symposium and live tweeted throughout the event (look for a ton of tweets from the Symposium under...the topics discussed during the symposium will prove incredibly important to the stability of the biological...takeaways I got from the symposium. The goal of the Future of Research Symposium On the FOR website, the...inclusion The final and possibly the most important panel of the symposium was on diversity in academic research...sponsored the Future of Research (FOR) Boston Symposium 2015. I personally participated as a member of...main question they wanted to focus on during the symposium was “How many graduate students/post-docs?” You... clear throughout the various sessions of the symposium. Structural problems in academic research and ...

...that company culture and enjoying your job were important parts of team buidling - they relieved some of...

...Policy Group to organize a Minisymposium on Reproducibility. The minisymposium focused on solutions to reproducibility...use computer based analysis techniques, an important component of reproducibility is making sure that others... both in preparation for my talk and at the minisymposium. You can find a variety of additional resources...findings can be predictably applied to future work. Importantly, no one should expect that every study should...scientific experts discussed by Pamela Hines Minisymposium on Reproducibility Booklet - Including descriptions...

...Orientation)) is a powerful tool that provides precise temporal and spatial control of gene expression in vivo...- or reward-related behaviors. The high spatio-temporal precision and reversible modulation of FLEx vectors...

...-hyg will be used to create gene deletions of important unknown regulatory regions in Pseudomonas and ...functions in biofilm formation. The quest for plant compounds to treat complications of diabetes Gloria Otito...Her goal is to identify and isolate pure plant compounds with antidiabetic properties that can ameliorate...expression systems here) and the activity of the plant compounds on these proteins will be tested. Investigating...

...different components, but all contain a FRET pair, a genetic component, and a small molecule component. Early...developed voltage sensor with a genetic component. The VSD components have historically come from a variety...cellular localization component is typically handled by either the fluorescent component (i.e., when the dye...tools have two main elements: a voltage-sensing component and a fluorescent output. The GEVI family of gadgets... a hybrid approach and utilize both a genetic component as well as a small molecule which we will describe...reporter and the mechanism of their voltage sensing component, which separates them into two distinct categories...squirts. This design structurally separates the two components of the indicator, which can make the kinetics...

...replication components are called helper plasmids. Below, we’ll breakdown the vector components first by ...nucleocapsid viral components needed for viral packaging, assembly, and display. Pol, the last component, contains...there are bonus components to these vectors that aren’t reflected in the common components outlined above...lentiviral guide outlines these components.     Fig. 1 - Viral vector components of retrovirus  Adeno-associated...production reasons, multiple plasmids with many components are used to package lab grown viruses. It can... protocols! In reality, there are many shared components across viruses (AAV, lentivirus, etc.) and their... what gag and pol stand for, we demystify all components of viral vectors here! Need Virus? Check out...

...compatible with all your components. Next, consider the best form for those CRISPR components based on your model...introducing those CRISPR components into your target cells. Delivery of these components into mammalian cell...most effectively achieved with different CRISPR components. Select the appropriate CRISPR tools for your...will you be editing? Do you want to deliver the components as DNA, or would mRNA or protein delivery be ...of the first steps is to identify what CRISPR components you will need to deliver. Minimally, one or more... the best method for introducing those CRISPR components into your target cells.  Mammalian CRISPR expression...best practices for efficient delivery of CRISPR components. If you are new to CRISPR or your model system...

...Science Symposium and Workshop 2016 – Sept 16-17 MIT, Boston The Advocating for Science Symposium and Workshop...Advocating for Science Symposium Boston 2016 Changes since the Future of Research Symposia Ongoing challenges...skills. Advocating for Science Symposium and Workshop 2016 At past symposia, FoR worked to highlight some...necessary for affecting change. On Friday, the symposium includes a panel discussion and keynote by former...eLife, 2016). Several Future of Research (FoR) symposia held across the USA during this time have revealed...the efficacy of their advocacy skills. Friday’s symposium will include a discussion on effective advocacy...Chief Diversity Officer Harvard University  The symposium will culminate with a keynote address by former...

... whether respondents saw them as not important to very important. In addition, we also explored the barriers... study, respondents were also asked about how important ease of use is for them (if they are to switch...ELN choice, with almost 80% rating it as very important. One comment reflected the desire for a flexible... category;”. The study also showed that It is important that an ELN supports all types of data, which ...data during their work. It is already becoming impossible to keep track of it all in paper notebooks. Following...supplement their paper notebooks. Instead, all of the important information is consolidated to one place, easily...paper records as well as electronic ones? It is important to know in which ways your data can be exported...

...sequence are important considerations for designing your gRNAs. For indels, it's not so important what location...nom. Me love gRNAs! Comic by Maya Kostman.   Important considerations before you start an experiment ...tool among them. While this seems obvious, it is important to remember that the same is true when designing...location in the gene you target, but it is important that your gRNA sequence is designed to be highly active...CRISPRi, these considerations are of roughly equal importance (target should be near the TSS but you can worry...from). Finally, for HDR, location is much more important because you have to target within ~30 nt of your...gRNA with an optimized sequence is of primary importance (more on this below).       The jigsaw: Editing...

...proteins are provided in trans (see Other Important Components of EBOV Minigenome System). However, minigenomes...sense RNA viruses (reviewed in 2, 3). Other important components of the Zaire ebola virus minigenome system...antiviral compounds which often target the viral polymerase. Technical tips It is important that all of...particular observation can be easily studied as each component can be examined independently. In addition, experiments...efficiently transfected into cells. All of these components are necessary to efficiently reconstitute a functional...utilize the minigenome as a template. Thus, it is important to choose cell lines that are highly transfectable...plasmid transfected into cells are also extremely important. For example, particular ratios of plasmids encoding...

...interacting proteins. No spatiotemporal control. Many PPI detection methods lack spatiotemporal control or an “...Split-BioID, a spatiotemporally controllable version of the BioID protocol. The critical component for both ... lysate fractionation. Important considerations for Split-BioID It’s important to remember that with Split-BioID...proteomics approach to monitor the composition of spatiotemporally defined protein complexes. Nature communications...this limitation by creating Split-BioID, a spatiotemporally controllable version of the proximity-dependent... other PPI methods, including BioID, is its spatiotemporal controlled labeling of interacting proteins...protein will be biotinylated. This makes it’s impossible to distinguish proteins that directly interact...

... out, cover letters are getting more and more important in the job recruiting process. Lauren Nelson, ...Cogency says: “A well-written cover letter is more important than an impressive résumé because it reveals your...Resumes Australia states: “A cover letter is as important as the resume.” David G. Jensen, a writer and ...best candidate for a position, it is extremely important not only to prepare a good resume/CV (listing ...help you grow professionally, it is very, very important to take some time and mentally reflect on what...you want to apply for a certain job,  the next important step is to do extensive research about the company...applying for PhD or postdoctoral positions, it is important to learn about the PI (if he/she is a good mentor...

...regulation, but enhancers and chromatin looping play important supporting roles. Enhancers are cis regulatory...of a stable cell line that co-expresses three components: dCas9 with an added biotin acceptor site BirA...genomic location of interest  When these three components are co-expressed, the gRNA targets the dCas9 ...with primary cells or rare cell populations. Key components of the CAPTURE 2.0 system To improve upon the... Using this tag reduces the number of CAPTURE components that need to be delivered to cells from three...lentiviral delivery of the two remaining CAPTURE 2.0 components eliminates the need to create stable cell lines...networks’ of 157 super enhancers.  3. Measuring temporal changes in chromatin interactions A third application...