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p3E EFS-rtTA/rtTA3 (JDW 1214)

Plasmid

#224533

Purpose

A Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivator

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rtTA

Use

Gateway cloning

Promoter

EFS

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

Plasmid

#224534

Purpose

A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mNeonGreen-HA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKP712/713A

Plasmid

#216544

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: P712/713A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (P712/713A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation P712/713A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKS732A/S910A

Plasmid

#221642

Purpose

This retroviral plasmid expresses a double-mutated cDNA (dominant negative: S732A/S910A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S732A/S910A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK double mutation S732A/S910A (dominant negativ…

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKR177/178A

Plasmid

#216543

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: R177/178A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (R177/178A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation R177/178A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(S131C/A286F)

Plasmid

#224775

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S131C / A286F

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131A/C159A/C219A)

Plasmid

#224753

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131A/C159A/C219A

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131C/C159A/C219A)

Plasmid

#224752

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C/C159A/C219A

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)

Plasmid

#224754

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

C159A/C219A/C365A/C399A

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-mTFP1

Plasmid

#223638

Purpose

Lentiviral expression of human CD28 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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