We narrowed to 594 results for: pFAST

1. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac Dual PNG172-STII PLU-HIS

   Plasmid

   #112992

   Purpose

   Baculovirus expression of Drosophila png172-Flag and plu-His

   Depositor

   Terry Orr-Weaver

   Inserts

   png172 (png Fly)
   plu (plu Fly)
   

   Use

   Tags

   Flag and His

   Expression

   Insect

   Mutation

   png172 mutation

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac Dual PNG-STII PLU-HIS

   Plasmid

   #112993

   Purpose

   Baculovirus expression of Drosophila png-Flag and plu-His

   Depositor

   Terry Orr-Weaver

   Inserts

   png (png Fly)
   plu (plu Fly)
   

   Use

   Tags

   Flag and His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBacHT (His10-4xFRB-SNAP-Lck G2A)

   Plasmid

   #177887

   Purpose

   Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Lck (LCK Human)
   

   Use

   Tags

   10xHis-TEV-4xFRB-SNAP tag

   Expression

   Insect

   Mutation

   G2A

   Promoter

   Polyhedrin

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1 human full-length CENP-E-mNeon-6xHis

   Plasmid

   #180484

   Purpose

   pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

   Depositor

   Julie Welburn

   Article

   Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

   Insert

   CENP-E (CENPE Synthetic, human, Human)
   

   Use

   Tags

   mNeon-6xHis

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac Dual His6 TEV LIC cloning vector (5B)

   Plasmid

   #30122

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

   Plasmid

   #79031

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(African green monkey pygerythrus)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

   Plasmid

   #79036

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Changed Lys 283 to Asp and Gln 287 to Asp

   Promoter

   polyhedrin

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac HTB His-TEV-rDPP9(S729A)-FLAG

   Plasmid

   #217329

   Purpose

   Purification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cells

   Depositor

   Yifan Wang

   Insert

   DPP9
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   S729A

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG

    Plasmid

    #217325

    Purpose

    Purification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    N61K

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

    Plasmid

    #217324

    Purpose

    Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    S969A

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

    Plasmid

    #217320

    Purpose

    Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

    Plasmid

    #55218

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac His6 MBP N10 TEV LIC cloning vector (4C)

    Plasmid

    #30116

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

    Plasmid

    #198035

    Purpose

    Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    EGFP and GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR)

    Plasmid

    #223778

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Insect

    Mutation

    W18A/L21A; deletion of LIR

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

    Plasmid

    #217321

    Purpose

    Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    S969A

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac dual_Human full length CDK7(W132R, S164D, T170E)

    Plasmid

    #217013

    Purpose

    Expression of CDK7 in insect cells

    Depositor

    Manjeet Mukherjee

    Article

    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

    Insert

    CDK7(W132R, S164D, T170E) (CDK7 Human)
    

    Use

    Tags

    TEV cleavable his tag

    Expression

    Insect

    Mutation

    CDK7(W132R, S164D, T170E)

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

    Plasmid

    #79030

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    deleted amino acid 294-515 from frican green monk…

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

    Plasmid

    #48294

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge