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pFUS_A4B

Plasmid

#43854

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaE

Plasmid

#228826

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaE

Tags

eGFP

Expression

Mammalian

Mutation

aa 49-494

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaD

Plasmid

#228825

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaD

Tags

eGFP

Expression

Mammalian

Mutation

aa 30-700

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaC

Plasmid

#228824

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

eGFP

Expression

Mammalian

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaA

Plasmid

#228823

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaA in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaA

Tags

eGFP

Expression

Mammalian

Mutation

aa 25-1216

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-STOP

Plasmid

#215840

Purpose

Expression of FLAG/HA tag

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

FLAG/HA

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRMCD111

Plasmid

#172957

Purpose

pRMCD77 encoding Wzz mutant G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G311A mutant gene

Expression

Bacterial

Mutation

D168G and N33D- please see depositor comment

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD113

Plasmid

#172959

Purpose

pRMCD77 encoding Wzz mutant G305A/G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G311A mutant gene

Expression

Bacterial

Mutation

G305A/G311A, A208V - please see depositor comments

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-VN155(I153L)-TRP1

Plasmid

#53193

Purpose

chromosomal tagging with Venus

Depositor

John Pringle

Article

Onishi et al J Cell Biol. 2013 Jul 22;202(2):311-29.

Insert

Venus

Use

Yeast genomic targeting

Tags

TRP1

Mutation

aa 1–155, I to L mutation at aa 153

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-VAMP8

Plasmid

#45912

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 8 (VAMP8 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pET29b-IPP1-His

Plasmid

#124137

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Inorganic pyrophosphatase (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-His-RF2

Plasmid

#124130

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 2 (prfB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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HyperNova/pRSETB

Plasmid

#221456

Purpose

Fast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivation

Depositor

Kiwamu Takemoto

Article

Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

Insert

HyperNova

Expression

Bacterial

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a-MTF-His

Plasmid

#124133

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionyl-tRNA formyltransferase (fmt Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGFPmAID

Plasmid

#174213

Purpose

GFP::mAID donor backbone

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Type

Empty backbone

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-VAMP7

Plasmid

#45913

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 7 (Vamp7 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET21a-MetRS-His

Plasmid

#124115

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionine--tRNA ligase (metG Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-HisRS-His

Plasmid

#124111

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Histidyl-tRNA ligase (hisS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-LysRS-His

Plasmid

#124114

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Lysine-tRNA ligase (lysU Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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