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pCMV-ClpX

Plasmid

#234733

Purpose

Human-codon optimized E. coli ClpX in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ClpX

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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HA-mios in pRK5

Plasmid

#154212

Purpose

N-terminal HA-tagged Mios cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

Mios

Tags

HA tag

Expression

Mammalian

Mutation

cDNA sequence has been codon optimized using huma…

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mEGFP-TSG101

Plasmid

#116925

Purpose

expresses mEGFP-TSG101 in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

TSG101 (TSG101 Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

Plasmid

#79671

Purpose

Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-WT

Plasmid

#133753

Purpose

Wild type human TDP-43 in pLVX-Puro lentiviral vector

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-mSTING

Plasmid

#229739

Purpose

Expresses mouse STING in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

STING (Sting1 Mouse)

Tags

None

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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xCas9 3.7

Plasmid

#108379

Purpose

Mammalian expression vector for xCas9 3.7

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.7

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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YFP-NOP56-C3

Plasmid

#134536

Purpose

Expresses YFP-NOP56 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

NOP56 (NOP56 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBP-T7_RBS-His6-Thrombin

Plasmid

#72979

Purpose

T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7+RBS+His6+thrombin

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
HA-GbL

Plasmid

#1865

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

GbL (MLST8 Human)

Tags

HA

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pGEMS-II-dCas13-APO1-YTH

Plasmid

#204767

Purpose

GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

dCas13-NLS-APO1-YTH-HA

Use

Synthetic Biology

Expression

Mammalian

Promoter

Ef1alpha

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-XPLN

Plasmid

#73375

Purpose

GST-tagged expression of XPLN (ARHGEF3) in bacteria

Depositor

Jie Chen, Krister Wennerberg

Article

Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

Insert

arhgef3 (ARHGEF3 Human)

Tags

GST

Expression

Bacterial

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-SAMTOR

Plasmid

#100508

Purpose

Expresses FLAG tagged SAMTOR in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRTH

Plasmid

#136925

Purpose

Expresses Cas9-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2797

Plasmid

#63639

Purpose

pCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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piRFP-N3-Tyrosinase

Plasmid

#80152

Purpose

Melanosome marker

Depositor

Santiago Di Pietro

Article

Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

Insert

Tyrosinase (TYR Human)

Tags

iRFP682

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3.1-hMcl-1

Plasmid

#25375

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pBS hShh (CT#401)

Plasmid

#13996

Depositor

Cliff Tabin

Article

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Shh (SHH Human)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pVETL-Cmcs-Flag-Ataxin3Q80

Plasmid

#22120

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pOPINS-AREL1

Plasmid

#66710

Purpose

Expresses AREL1 HECT domain in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KIAA0317 (AREL1 )

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only