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Addgene

We narrowed to 8,031 results for: gal

Showing: 6101 - 6150 of 8031 results
  1. pLCHKOv3-CD46 Ex3_3-As

    Plasmid
    #209033
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Insert
    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pGBT9-FHIP

    Plasmid
    #198527
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Insert
    FHIP (FHIP1B Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    silent mutations in codons 353 and 651 as well as…
    Promoter
    ADH1
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pGBT9-Hook1

    Plasmid
    #198523
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Insert
    Hook1 (HOOK1 Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    silent substitution in codon 620 to eliminate int…
    Promoter
    ADH1
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pGL3-pSMANTIS_SMAD-FOS_Mutant

    Plasmid
    #194188
    Purpose
    Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated SMAD/FOS Site, Region 3-15nt of 1000nt
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pGL3-pSMANTIS_STAT1-2_Mutant

    Plasmid
    #194189
    Purpose
    Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated STAT1/2 site, Region 653-673nt of 1000nt
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pIFEX-A2aGH

    Plasmid
    #160542
    Purpose
    pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pEFEX1-A2aGH

    Plasmid
    #160543
    Purpose
    pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pEFEX2-A2aGH

    Plasmid
    #160544
    Purpose
    pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Insert
    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pEFEX3-A2aGH

    Plasmid
    #160545
    Purpose
    pYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Insert
    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pCFEX1-A2aGH

    Plasmid
    #160546
    Purpose
    pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pCFEX2-A2aGH

    Plasmid
    #160547
    Purpose
    pYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Insert
    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pCFEX3-A2aGH

    Plasmid
    #160548
    Purpose
    pYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Insert
    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pCSmEn2ERT(#117)

    Plasmid
    #184079
    Purpose
    cyclofen-inducible chicken EN2 (wt) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Insert
    6his-myc-chkEn2-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pCSm5En2CER4(#457)

    Plasmid
    #184090
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Insert
    5myc-chkEn2(C>S)-ERT2
    Use
    Synthetic Biology
    Tags
    5xMyc
    Expression
    Mammalian
    Mutation
    chkEn2: C175S; ERT2: G400V, M543A, L544A, deleted…
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. MIOX only

    Plasmid
    #166681
    Purpose
    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Insert
    MIOX (Miox Budding Yeast, Synthetic, Mouse)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pcDNA3-sPDGFRalpha-Fc (Legacy)

    Plasmid
    #136437
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
    Depositor
    Insert
    PDGFRalpha (PDGFRA Human)
    Use
    Tags
    Fc region of IgG1 (a.a. Pro-217 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524)
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  17. EcNGsC-DelFSH(307-309)-pKK223

    Plasmid
    #131377
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.
    Depositor
    Insert
    EcNGsC MutY chimera DelFSH(307-309)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are from…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  18. EcNGsC-S308P-H309D-pKK223

    Plasmid
    #131378
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.
    Depositor
    Insert
    EcNGsC MutY chimera S308P H309D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are from…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. EcNGsC-F307A-S308A-pKK223

    Plasmid
    #131379
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.
    Depositor
    Insert
    EcNGsC MutY chimera F307A S308A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are fro…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. pABD823

    Plasmid
    #67911
    Purpose
    Protein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae
    Depositor
    Insert
    Cold temperature germinating 10 (CTG10)
    Use
    Tags
    GAL4 DNA binding domain (148 amino acids)
    Expression
    Yeast
    Mutation
    Promoter
    pADH1 (alcohol dehydrogenase 1 promoter)
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 6101 - 6150 of 8031 results