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pBAD_LcgcC-GFP-H6

Plasmid

#245528

Purpose

His-tagged superfolder GFP C-terminally fused to C-terminal LCGC14 split intein fragment

Depositor

Henning Mootz

Article

Humberg et al Angew Chem Int Ed Engl. 2025 Oct 23:e202

Insert

C-terminal Fragment of the LCGC14 PolB2 Split Intein

Tags

Hexahistidine Tag and Superfolder Green Fluoresce…

Expression

Bacterial

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pZNp-2

Plasmid

#245373

Purpose

Negative control, firefly luciferase mutated

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZ_pF-3

Plasmid

#245374

Purpose

Negative control, NanoLuciferase mutated

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆7

Plasmid

#245367

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆7 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

7-bp deletion, H96Vfs*29

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆8

Plasmid

#245368

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆8 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

synthetic 8-bp deletion

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-wt

Plasmid

#245364

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆59

Plasmid

#245365

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆59 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

59-bp deletion, D78Vfs*6

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆11

Plasmid

#245366

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆11 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

11-bp deletion, H96Wfs*4

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-aex-5-flag-tev-halo-his

Plasmid

#227152

Purpose

For T-REX experiments of aex-5.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

aex-5 (aex-5 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-asp-2-flag-tev-halo-his

Plasmid

#227153

Purpose

For T-REX experiments of asp-2.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

asp-2 (asp-2 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_THRAP3_THSD7A fusion protein LCD (663-955)

Plasmid

#235002

Purpose

Bacterial expression of N-terminally 6His tagged THRAP3_THSD7A (THRAP3) fusion protein LCD

Depositor

Tanja Mittag

Insert

THRAP3_THSD7A (THRAP3) (Thyroid hormone receptor-associated protein) (THRAP3, THSD7A Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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p8224 pLIX-PTPN14 ΔPPXY1/2

Plasmid

#221644

Purpose

Doxycycline-inducible overexpression of human PTPN14 ΔPPXY1/2 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY1/2 (PTPN14 Human)

Use

Lentiviral

Tags

HA

Mutation

deletion of the first and second (tandem) PPXY mo…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8552 pLIX-PTPN14 ΔPPXY3/4

Plasmid

#221645

Purpose

Doxycycline-inducible overexpression of human PTPN14 ΔPPXY3/4 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY3/4 (PTPN14 Human)

Use

Lentiviral

Tags

HA

Mutation

deletion of the third and fourth PPXY motifs as d…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8215 pHAGE N-V5 PTPN14 ΔPPXY1/2

Plasmid

#221646

Purpose

Constitutive ectopic expression of human PTPN14 ΔPPXY1/2 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY1/2 (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

deletion of the first and second (tandem) PPXY mo…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8180 pHAGE N-V5 PTPN14 ΔPPXY3/4

Plasmid

#221647

Purpose

Constitutive ectopic expression of human PTPN14 ΔPPXY3/4 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY3/4 (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

deletion of the third and fourth PPXY motifs as d…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8189 pHAGE N-V5 PTPN14 C1121S

Plasmid

#221648

Purpose

Constitutive ectopic expression of human PTPN14 C1121S mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 C1121S (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

C1121S mutation at the putative catalytic site

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8391 LentiCRISPRv2 Neo sgPTPN14-1

Plasmid

#221651

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-1 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8392 LentiCRISPRv2 Neo sgPTPN14-3

Plasmid

#221652

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-3 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AP-SpyCatcher

Plasmid

#223636

Purpose

Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His10, AP fragment of split APEX2 tag

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher -AVI

Plasmid

#223635

Purpose

Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

AVI tag and His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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