We narrowed to 16,297 results for: nans

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araBAD-BCD20-ClpP-BCD17-ClpX

Plasmid

#153301

Purpose

Expresses clpX and clpP in E.Coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Inserts

clpX (clpX Escherichia coli)
clpP (clpP Escherichia coli)

Expression

Bacterial

Promoter

araBAD

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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SUPERBLADE5

Plasmid

#134913

Purpose

Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Promoter

U6

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-pAce-Kv2.1PR

Plasmid

#195529

Purpose

Green fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

CaMKII

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Ace-mNeon2-Kv2.1PR

Plasmid

#195522

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

Synapsin

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-TRE-AFOS

Plasmid

#197079

Purpose

A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

Depositor

Elaine Fuchs

Article

Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

Insert

AFOS (Fos Mouse)

Use

Lentiviral

Tags

Flag and T7

Promoter

TRE

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB6

Plasmid

#200984

Purpose

Mammalian expression plasmid for myc-tagged EphB6

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB6 (EPHB6 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-Laconic/pCMV-myc-nuc

Plasmid

#46307

Purpose

a nuclear-targeted, genetically-encoded sensor for lactate

Depositor

Luis Felipe Barros

Article

Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

Insert

NLS-Laconic

Use

Lactate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-3x-p67phox-GBD

Plasmid

#214141

Purpose

Rac activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xp67phox-GBD (NCF2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-pAce-Kv2.1PR

Plasmid

#195535

Purpose

Flp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLpA-DN.JNK1

Plasmid

#51942

Purpose

Adenoviral vector for dominant negative JNK1 expression

Depositor

Jeffery Molkentin

Article

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

DN JNK1 (Mapk8 Mouse)

Use

Adenoviral

Tags

FLAG

Mutation

dominant negative: the dual phosphorylation moti…

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-DIO-pAceR-Kv2.1PR

Plasmid

#195534

Purpose

Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-Ace-mNeon2-Kv2.1PR

Plasmid

#195532

Purpose

Cre-dependent expression of a green fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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L2096-GPA-BRET-Biosensor-Retrovirus-TD146

Plasmid

#222878

Purpose

Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAce-Kv2.1PR

Plasmid

#195523

Purpose

Green fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

Synapsin

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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(344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

Plasmid

#168097

Purpose

HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

HA-nGFP-(EAAAK)4-OGA(544-706)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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BLADE-182

Plasmid

#134914

Purpose

sgRNA targeting GFP to be used in nanoblade system

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GFP

Use

CRISPR and Synthetic Biology

Promoter

U6

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-s-pep

Plasmid

#47438

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Article

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-LOV*-MVP

Plasmid

#202206

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Major vault protein (MVP Human)

Tags

FLAG and LOV*

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3xVHH05-DsRed

Plasmid

#171580

Purpose

For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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(SUMO)5-(SIM)10

Plasmid

#126923

Purpose

Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Mutation

internal BamHI site between (SUMO)5 and (SIM)10

Promoter

tac

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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