We narrowed to 4,033 results for: pCMV mammalian

1. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-SEAP

   Plasmid

   #210513

   Purpose

   expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON and SEAP reporter in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Inserts

   secreted alkaline phosphatase
   CMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and TetO

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c

   Plasmid

   #210508

   Purpose

   expresses NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c
   

   Use

   Tags

   GB1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-NTOM20-EGFP-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs

   Plasmid

   #210516

   Purpose

   expresses NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP

   Plasmid

   #210505

   Purpose

   expresses NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

   Plasmid

   #176459

   Purpose

   Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

   Plasmid

   #176460

   Purpose

   Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

   Plasmid

   #217797

   Purpose

   Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…

   Promoter

   CMV and T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

   Plasmid

   #217803

   Purpose

   Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…

   Promoter

   CMV and T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16

   Plasmid

   #210506

   Purpose

   expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16 component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p2xSTAT6-Luc2P

    Plasmid

    #35486

    Depositor

    Axel Nohturfft

    Insert

    STAT6 promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2x Stat6-c/EBP

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS2402-Tier1-PhCMV-BlastR

    Plasmid

    #169494

    Purpose

    Tier-1 vector encoding PhCMV-driven blasticidin resistance gene BlastR (PhCMV-BlastR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven blasticidin-S deaminase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTS1021-Tier1-PhCMV-PuroR

    Plasmid

    #169492

    Purpose

    Tier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven puromycin N-acetyl-transferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p4xSTAT6-Luc2P

    Plasmid

    #35554

    Depositor

    Axel Nohturfft

    Insert

    4xStat6 promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    4xStat6

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTS2404-Tier1-PhCMV-NeoR

    Plasmid

    #169496

    Purpose

    Tier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven aminoglycoside 3'-phosphotransferase, APH 3' II
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3805

    Plasmid

    #198147

    Purpose

    Mammalian expression of LAMP1-miniSOG

    Depositor

    Michael Hensel

    Insert

    PCMV::LAMP1::miniSOG::mCherry (LAMP1 Human)
    

    Use

    Tags

    miniSOG-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3806

    Plasmid

    #198148

    Purpose

    Mammalian expression of LAMP1-miniSOG

    Depositor

    Michael Hensel

    Insert

    PCMV::LAMP1::tdminiSOG::mCherry (LAMP1 Human)
    

    Use

    Tags

    tdminiSOG-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDN-D2irC6kwh

    Plasmid

    #44725

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    mCherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDN-D2irG4kwh

    Plasmid

    #44723

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    EGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF1a-PEmax-P2A-GFP

    Plasmid

    #202000

    Purpose

    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.

    Depositor

    Daniel Geschwind

    Insert

    PEmax-P2A-GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDN-D2irTN6kwh

    Plasmid

    #44724

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    htetR::NLS
    

    Use

    Synthetic Biology; Expression regulator/reporter;…

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge