We narrowed to 5,570 results for: donor
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pEN_TTmiRC2_3xflag_Luciferase
Plasmid#136519PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertLuciferase
UseEntry vector for gateway cloningTags3x FLAGAvailabilityAcademic Institutions and Nonprofits only -
pFETCh_RXRB
Plasmid#104040PurposeDonor vector for 3' FLAG tag of human RXRBDepositorAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG_IGF1R_EGFP
Plasmid#199628PurposeFRET donor for IGF1R sensorDepositorAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-Rac1
Plasmid#83950PurposeRac1 FLIM donor for dynamic imaging of GTPase activityDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
TrkB mEGFP
Plasmid#83952PurposeFRET donor for dynamically measuring TrkB activity in neuronsDepositorAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR
Plasmid#126705Purposedonor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertGFP
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Puro-CAG-mNG2(1-10)
Plasmid#206042PurposeRepair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.DepositorInsertAAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
UseCRISPR and Synthetic Biology; Donor templateExpressionMammalianAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT077
Plasmid#137879PurposeDonor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFPDepositorInsertTRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
UseAAVExpressionMammalianAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_CAG-ITPKA-3x-mScarlet-I
Plasmid#229782PurposeBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)DepositorInsert3x mScarlet-I
UseSynthetic BiologyTagsITPKAExpressionMammalianPromoterCAGAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT076
Plasmid#137880PurposeDonor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFPDepositorInsertTRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
UseAAVExpressionMammalianAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-idCas9-vpr
Plasmid#89985Purposeinducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locusDepositorInsertdCas9-VPR
ExpressionMammalianPromoterTRE-tightAvailable SinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
PECAM1-mRuby3-T2A-secNluc
Plasmid#235607PurposeDonor plasmid for knock-in of mRuby3-T2A-secNluc into the human PECAM1 locusDepositorInsertPECAM1 homologous recombination arms with PuroR drug selection cassette
UseCRISPRAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyExpressionMammalianPromoterEF1α and pTRE3GAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDOC-GG
Plasmid#149377PurposeChassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene DoctoringDepositorInsertsFragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
Plasmid#196192Purposehuman synapsin promoter driven dTomato expression at human AAVS1 safe harbour locusDepositorInsert2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
UseZnf donorAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only
