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We narrowed to 3,093 results for: FRI

Showing: 2501 - 2520 of 3093 results

  1. pCUU

    Plasmid
    #159747
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159747-plasmid-map-sequence-id-313815

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  3. ORAI1-YFP V107M-L273D-L276D

    Plasmid
    #114183
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    V107M/L273D/L276D
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114183-plasmid-map-sequence-id-218685

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  4. ORAI1-YFP T184M-L273D-L276D

    Plasmid
    #114184
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    T184M/L273D/L276D
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114184-plasmid-map-sequence-id-220961

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  13. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

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  15. GABA(A) receptor subunit a2SE

    Plasmid
    #49169
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    Use
    Tags
    HA, bovine alpha 1 signal sequence, and pHGFP (pH…
    Expression
    Mammalian
    Mutation
    T343A
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49169-plasmid-map-sequence-id-72495

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  17. pDule-3-nitroTyrosine (A7)

    Plasmid
    #174078
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174078-plasmid-map-sequence-id-345712

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  18. pICE-HA-Ku70-siR-Mut6E

    Plasmid
    #82330
    Purpose
    Plasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10
    Insert
    Ku70 (XRCC6 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Mut6E corresponding to K282E K287E T300E K331E K3…
    Promoter
    CMV-tet
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    82330-plasmid-map-sequence-id-155730

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  19. Cherry-HA-KCC2

    Plasmid
    #104077
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Article
    Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Use
    Tags
    mCherry-HA tag
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104077-plasmid-map-sequence-id-199357

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  20. B2M Bulldozer (gRNA crB2M_13)

    Plasmid
    #84381
    Purpose
    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells
    Depositor
    Chad Cowan
    Article
    Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.
    Insert
    B2M Bulldozer crB2M_13 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 promoter
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84381-plasmid-map-sequence-id-189203

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Showing: 2501 - 2520 of 3093 results