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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,974 results for: codon optimized

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Showing: 2501 - 2520 of 4974 results

  1. pfMDH_Triple

    Plasmid
    #67733
    Purpose
    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Publication
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine, arginine 373 to…
    Promoter
    T7
    Available since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pHS1543

    Plasmid
    #205963
    Purpose
    NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    DinG HNH Cascade with His14-tagged Csf3
    Tags
    14xHis on Csf3
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205963-plasmid-map-sequence-id-415370

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  6. pLV_PP1-mCitrine

    Plasmid
    #172472
    Purpose
    Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.
    Depositor
    Sean R Collins
    Publication
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Insert
    prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
    Use
    Lentiviral
    Tags
    mCitrine and prolactin signal sequence (cleaved p…
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172472-plasmid-map-sequence-id-342800

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  7. pCEP4-HLA-c-myc-optiT1R3 ECD

    Plasmid
    #113960
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Publication
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113960-plasmid-map-sequence-id-215290

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  9. p-mCherry-C1-iChR2++

    Plasmid
    #98172
    Purpose
    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Synthetic construct iChR2++ gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98172-plasmid-map-sequence-id-182770

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  15. pSW002-Pc-TorA(sp)-mNeonGreen

    Plasmid
    #205020
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Publication
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorA-mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205020-plasmid-map-sequence-id-407993

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  16. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

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  18. SpCas9-YE1-BE4max

    Plasmid
    #198546
    Purpose
    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198546-plasmid-map-sequence-id-390295

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  19. SpCas9-NG-SsAPOBEC3B

    Plasmid
    #198536
    Purpose
    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-SsAPOBEC3B
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198536-plasmid-map-sequence-id-388885

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  20. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

    Plasmid
    #208290
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpCas9(D10…
    Promoter
    CMV and T7
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208290-plasmid-map-sequence-id-410848

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Showing: 2501 - 2520 of 4974 results