Addgene

We narrowed to 6,106 results for: cat.2

Showing: 2521 - 2540 of 6106 results

Results per page:

Sort results by:

pcDNA5-FRT-TO-FH-Nprot

Plasmid

#157729

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nprot (N SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
NSP14-Flag

Plasmid

#210346

Purpose

Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP14 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NSP4-FR-2Strep

Plasmid

#210316

Purpose

Expresses SARS-CoV-2 NSP4 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-FRT-TO-Nprot_GC3opt

Plasmid

#157730

Purpose

mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot_GC3opt (N SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
P1-M-P2

Plasmid

#87091

Purpose

This is the P1-M-P2 portion of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmid

Depositor

Yohannes Tesfaigzi

Article

Bredow et al Gene. 2007 Dec 1;404(1-2):110-6. Epub 20

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
Bn R52A, R54A pMT2

Plasmid

#206087

Purpose

Cav2.2 N-terminus (amino acids 1-95) with R52A and R54A mutations that prevent dominant-negative suppression of Cav2.2 currents by truncated Cav2.2

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

R52A, R54A

Promoter

Ad MLP/TPL/SV40

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-optimizedNedd4.2d

Plasmid

#165103

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

Insert

Nedd4.2d (NEDD4L Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1-nsp7

Plasmid

#201026

Purpose

Expresses SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-nsp7

Plasmid

#201025

Purpose

Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-tet-ON

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NSP4-WT-Flag

Plasmid

#210339

Purpose

Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NSP4-Δ220-234-Flag

Plasmid

#210341

Purpose

Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Tags

Flag

Expression

Mammalian

Mutation

Δ220-234

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NSP4-H120N/F121L-Flag

Plasmid

#210340

Purpose

Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Tags

Flag

Expression

Mammalian

Mutation

H120N/F121L

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NSP4-mCherry

Plasmid

#210319

Purpose

Expresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Article

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Use

Tags

14Histidine-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Nprot-HF_GC3opt

Plasmid

#157732

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot-HF_GC3opt (N SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Promoter

Available since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2)

Plasmid

#223782

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BCL2L13 soluble part (BCL2L13 Human)

Use

Tags

EGFP-TEV-GST

Expression

Bacterial

Mutation

Y213A/I216A/W276A/I279A; deletion of LIR1+2

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pR

Plasmid

#193746

Purpose

Expresses LuxR under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-

Insert

LuxR

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23102

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NSP3-FR-2Strep

Plasmid

#210315

Purpose

Expresses SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3 (ORF1ab SARS-CoV-2)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1-nsp7-FLAG

Plasmid

#201027

Purpose

Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

8xHis and FLAG

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Nsp1

Plasmid

#157679

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge