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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,839 results for: pcr

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Showing: 2541 - 2560 of 4839 results

  1. pJK614

    Plasmid
    #72387
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 4 silent mutations in codons 9-11 and 42…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72387-plasmid-map-sequence-id-133927

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  12. GLRX3

    Plasmid
    #39036
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GLRX3 (GLRX3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    A PCR artifact adds a C-terminal sequence includi…
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pDONR221-SLC52A2_STOP

    Plasmid
    #161068
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC52A2 (GPR172A Human)
    Expression
    Mammalian
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    161068-plasmid-map-sequence-id-333112

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  15. pENTR-D-kcnj16

    Plasmid
    #173146
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Publication
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj16a (kcnj16 Zebrafish)
    Use
    Pcr cloning vector
    Mutation
    Stop codon removed
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173146-plasmid-map-sequence-id-343336

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  18. SEMA3D (mouse)

    Plasmid
    #29452
    Depositor
    Jonathan Raper
    Insert
    SEMA3D (Sema3d Mouse)
    Use
    For ish
    Mutation
    insert is not full length; contains multiple poin…
    Available since
    Feb. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  20. GPR61-Tango

    Plasmid
    #66366
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR61 (GPR61 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 2541 - 2560 of 4839 results