We narrowed to 2,799 results for: fas

1. pFB1.HMBP.PrS.PHF19

   Plasmid

   #125168

   Purpose

   Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   PHF19 (PHF19 Human)
   

   Tags

   His-MBP

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFBD-Akt13C(S473D)

   Plasmid

   #86577

   Purpose

   Expresses cleavable human full-length Akt1(S473D) phosphomimetic

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130; S473D phos…

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

   Plasmid

   #35512

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

   Plasmid

   #35500

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

   Plasmid

   #218866

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8f
   

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available Since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcBac1-GFP(39TAG)

   Plasmid

   #217360

   Purpose

   Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   Enhanced green fluorescent protein
   

   Use

   Baculoviral

   Expression

   Insect and Mammalian

   Mutation

   Y39TAG

   Promoter

   CMV

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

   Plasmid

   #218864

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8s
   

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   S26M F286Y Q315H

   Promoter

   hsp70

   Available Since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa-hChR2(E123A)-EYFP

   Plasmid

   #35505

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Has Service

   AAV9

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

   Plasmid

   #78509

   Purpose

   Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   CD45R0Ex (PTPRC Human)
   

   Tags

   His10 and SNAPf

   Expression

   Insect

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHW581

    Plasmid

    #198818

    Purpose

    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218867

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid

    #218865

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8s
    

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO hChR2(E123A)-mCherry

    Plasmid

    #35508

    Purpose

    AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-EphB4-Fc

    Plasmid

    #200986

    Purpose

    Mammalian expression plasmid for soluble EphB4 fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB4 (EPHB4 Human)
    

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Soluble ectodomain of EphB4

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. R

    Plasmid

    #167170

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-R
    ATP1B1
    

    Expression

    Bacterial and Insect

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. OXSM_pLX307

    Plasmid

    #98357

    Purpose

    Lentiviral expression of OXSM

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    OXSM (OXSM Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MIS18A_pLENTI-CAG-IRES-GFP

    Plasmid

    #176996

    Purpose

    Mammalian lentiviral expression vector encoding MIS18A

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    MIS18A (MIS18A Human)
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. S

    Plasmid

    #167172

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S
    ATP1B1
    

    Expression

    Bacterial and Insect

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ADAP1

    Plasmid

    #163905

    Purpose

    Full length mouse ADAP1 for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFB-Flag-MyoD(QQQ)-E12

    Plasmid

    #26003

    Depositor

    Jeffrey Dilworth

    Article

    Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

    Insert

    MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)
    

    Use

    Baculovirus transfer vector

    Tags

    Flag

    Mutation

    Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

    Available Since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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