We narrowed to 12,861 results for: sequence

1. Flag‐mTim1 (Bl/6)

   Plasmid

   #49205

   Purpose

   expresses Flag tagged mouse Tim1

   Depositor

   Lawrence Kane

   Article

   de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

   Insert

   Tim1 (BL/6 allele) (Havcr1 Mouse)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   Tim1 lacks its own signal sequence

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pEntry_HCoV-229E-nsp12

   Plasmid

   #168969

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-229E-nsp12 (HCoV229Egp1 )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEntry_HCoV-HKU1-nsp12

   Plasmid

   #168909

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-HKU1-nsp12 (ORF1ab )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEntry_SARS-CoV-1-nsp12

   Plasmid

   #168861

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   SARS-CoV-1-nsp12 (ORF1ab )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant

   Plasmid

   #149507

   Purpose

   Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   MFSD12 (MFSD12 Human)
   

   Use

   Transient expression

   Tags

   TEV-3XHA-3XFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized Sequence, p.LL256-257AA

   Promoter

   CMV

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEntry_HCoV-NL63-Nucleocapsid

   Plasmid

   #168926

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-NL63-Nucleocapsid (HCNV63gp6 )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LAMP1-bio-His

   Plasmid

   #51809

   Purpose

   Expresses full-length Lysosome-associated membrane glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EF1a_KLF9_P2A_Hygro_Barcode

   Plasmid

   #120495

   Purpose

   Barcoded lentiviral vector to express KLF9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF9 (KLF9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB-RTP1S-Integration

   Plasmid

   #129458

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP1 (Rtp1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Removal of the first 36 amino acids of the protein

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF1a_MYC Ser62Ala_P2A_Hygro_Barcode

    Plasmid

    #120514

    Purpose

    Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC Ser62Ala (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutation changing Serine to Alanine at amin…

    Promoter

    EF1a

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EF1a_KLF11_P2A_Hygro_Barcode

    Plasmid

    #120497

    Purpose

    Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF11 (KLF11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBT-R16

    Plasmid

    #31827

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    mini Tol2 ITR
    

    Use

    Cre/Lox; Gene trap

    Tags

    mRFP

    Expression

    Mutation

    mRFP lacks start codon

    Promoter

    beta Actin

    Available since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HNRNPH2_3xRRM_pGEX

    Plasmid

    #135115

    Purpose

    Recombinant expression of HNRNPH2 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    HNRNPH2 (HNRNPH2 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LRRC-bio-His

    Plasmid

    #53333

    Purpose

    Expresses full-length Leucine-rich repeat-containing protein 32 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    LRRC (LRRC32 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFIN-EF1a-GFP:2A:CHER(HA)-WPRE

    Plasmid

    #44597

    Depositor

    Susan Semple-Rowland

    Article

    Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371

    Insert

    EF1a-GFP:2A:CHER(HA)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human elongation factor -1a (pos 2528-3971)

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR223 HCoV-NL63 E_stop

    Plasmid

    #168684

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    E (HCNV63gp4 )
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pDONR223 HCoV-NL63 E_nostop

    Plasmid

    #168666

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    E (HCNV63gp4 )
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pEntry_HCoV-NL63-Envelope

    Plasmid

    #168924

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-NL63-Envelope (HCNV63gp4 )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ILF2_DZF_pGEX

    Plasmid

    #135162

    Purpose

    Recombinant expression of ILF2 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    ILF2 (ILF2 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3 2198

    Plasmid

    #49347

    Purpose

    This is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.

    Depositor

    Stephen Harris, Melissa Rogers

    Article

    Feng et al Biochim Biophys Acta. 1997 Jan 3;1350(1)

    Insert

    Bmp2 promoter (shorter sequence) (BMP2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Bmp2 promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge