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pM-mOrange2

Plasmid

#228627

Purpose

Mammalian expression of mOrange2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Publication

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mOrange2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mRuby3

Plasmid

#228628

Purpose

Mammalian expression of mRuby3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Publication

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mRuby3

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(W50D)

Plasmid

#188639

Purpose

For expression of human ATG13(W50D)

Depositor

Michael Lazarou

Publication

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Changed tryptophan 50 to aspartic acid

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ230-D156R

Plasmid

#188542

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing

Depositor

Yiping Qi

Publication

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-D156R

Use

CRISPR; Gateway compatible lbcas12a-d156r entry c…

Tags

NLS

Expression

Plant

Mutation

D156R

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-HsNRF2 (NFE2L2)

Plasmid

#194302

Purpose

Multisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRS

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

NFE2L2 (NFE2L2 Human)

Use

Multisite gateway entry vector

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R1CiM_4EGFP_R3EGFPi2

Plasmid

#185968

Purpose

Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

Depositor

Sangram Bagh

Publication

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Molecular IMPLY gate

Use

Synthetic Biology

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_R4187C

Plasmid

#187141

Purpose

Entry clone

Depositor

Eric Fischer

Publication

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

R4187C

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_F3194S

Plasmid

#187139

Purpose

Entry clone

Depositor

Eric Fischer

Publication

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

F3194S

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_H669Q

Plasmid

#187137

Purpose

Entry clone

Depositor

Eric Fischer

Publication

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

H669Q

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_H3962D

Plasmid

#187130

Purpose

Entry clone

Depositor

Eric Fischer

Publication

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

H3962D

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#1

Plasmid

#174323

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Publication

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2 (NKD2 Human)

Use

Lentiviral

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ288 (BoCas12a)

Plasmid

#138125

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BoCas12a without promoter

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

BoCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ286 (CMaCas12a)

Plasmid

#138122

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized CMaCas12a without promoter

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

CMaCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K

Plasmid

#112647

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Publication

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K

Expression

Yeast

Mutation

R54K

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJK663

Plasmid

#71612

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tyrosine-154 to phenylalanine, tryptophan…

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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CS2-HA-dASUN

Plasmid

#47041

Purpose

Expression of tagged-dASUN

Depositor

Laura Lee

Publication

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (asun Fly)

Tags

HA

Expression

Mammalian

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
pEMS1386

Plasmid

#29185

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple117 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1389

Plasmid

#29188

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple120 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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N-mCherry-TMEM230

Plasmid

#255856

Purpose

Expression of human TMEM230 (isoform 1), codon optimised, in mammalian cells. N-terminally tagged with mCherry

Depositor

Joseph Lyons

Publication

Scholtissek et al J Membrane Biol 259, 32 (2026)

Insert

TMEM230 (TMEM230 Human)

Tags

Flag-tag and N-mCherry with 3C cleavage site

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2026

Availability

Academic Institutions and Nonprofits only

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C-mCherry-TMEM230

Plasmid

#255857

Purpose

Expression of human TMEM230 (isoform 1), codon optimised, in mammalian cells, C-terminally tagged with mCherry

Depositor

Joseph Lyons

Publication

Scholtissek et al J Membrane Biol 259, 32 (2026)

Insert

TMEM230 (TMEM230 Human)

Tags

C-mCherry with 3C cleavage site and Flag-tag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2026

Availability

Academic Institutions and Nonprofits only

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