We narrowed to 9,409 results for: try

1. pCK083 desmaMCS:minpromEGFP,exorh:mCherry

   Plasmid

   #200016

   Purpose

   Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. opto-Tiam1

   Plasmid

   #174508

   Purpose

   Expresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   opto-Tiam1-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR223-BZLF2

   Plasmid

   #195784

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BZLF2

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BZLF2 (BZLF2 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. S1-Fc

   Plasmid

   #164220

   Purpose

   Expresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporter

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

   Insert

   Spike S1 domain (S SARS-CoV)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMito-MtrxRFP2

   Plasmid

   #172324

   Purpose

   fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

   Depositor

   Huiwang Ai

   Article

   Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

   Insert

   mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
   

   Use

   Tags

   His-tag and Mitochondria localization sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-MXT GIRK2

   Plasmid

   #20675

   Depositor

   Henry Lester

   Article

   Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

   Insert

   Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
   

   Use

   Xenopus expression

   Tags

   3' UTR Xenopus B- globin and 5' UTR Xen…

   Expression

   Mutation

   A369V

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMuLE_ENTR_TOP-NLuc1.1_L5-L4

   Plasmid

   #113867

   Purpose

   Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   TOP (wnt dependent promoter)
   NLuc
   

   Use

   Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. 791 p3E-2A-pCS2MCSpA v2

   Plasmid

   #195973

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-multiple cloning site
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJCC_076 AsCas12a R1226A

   Plasmid

   #171669

   Purpose

   For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   AsCas12a R1226A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginine 1226 to alanine

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E-pth2

    Plasmid

    #191428

    Purpose

    Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)

    Depositor

    Timothy Erickson

    Article

    Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi

    Insert

    pth2 regulatory region (pth2 Zebrafish)
    

    Use

    Tol2 gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSecTag-T210A-ATX

    Plasmid

    #17840

    Depositor

    Steven Rosen

    Article

    Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

    Insert

    T210A-ATX (ENPP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pRSFDuet1-His-TEV-OTC_Ub

    Plasmid

    #172134

    Purpose

    Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

    Depositor

    Shang-Te Danny Hsu

    Article

    Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

    Insert

    E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA HisB TO rFez1

    Plasmid

    #17385

    Depositor

    Stephen Goff

    Article

    Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

    Insert

    FEZ1 (Fez1 Rat)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pENTR221-NFAT5

    Plasmid

    #79506

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    NFAT5 (NFAT5 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMX-hTAF7

    Plasmid

    #44338

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF7 (TAF7 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. hDAGLb-V5

    Plasmid

    #87677

    Purpose

    Expresses human DAGLb protein with intracellular V5 tag located on the C-terminal

    Depositor

    Pat Doherty

    Article

    Singh et al Biochemistry. 2016 May 17;55(19):2713-21

    Insert

    Human Diacylglycerol Lipase Beta (DAGLB Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pirespuro2-CD86-mEos2

    Plasmid

    #98284

    Purpose

    mammalian expression of CD86-mEos2

    Depositor

    Mike Heilemann

    Article

    Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

    Insert

    CD86 (CD86 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR1A hu_ncOGT WT

    Plasmid

    #154281

    Purpose

    Entry vector encoding non-tagged O-GlcNAc Transferase

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    WT OGT

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK081 desmaMCS:minpromCerulean,exorh:mCherry

    Plasmid

    #200014

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMX-hTAF10

    Plasmid

    #44340

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF10 (TAF10 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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