We narrowed to 9,753 results for: try

1. dCas9-10xSunTag(22aa)

   Plasmid

   #215745

   Purpose

   Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   dCas9-10xSunTag
   

   Expression

   Mammalian

   Promoter

   chicken beta-actin promoter

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-MXT GIRK2

   Plasmid

   #20675

   Depositor

   Henry Lester

   Article

   Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

   Insert

   Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
   

   Use

   Xenopus expression

   Tags

   3' UTR Xenopus B- globin and 5' UTR Xen…

   Mutation

   A369V

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR221-NR1H2

   Plasmid

   #79513

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   LXR BETA (NR1H2 Human)
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. ND4.2-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #179683

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Expression

   Mammalian

   Mutation

   E1347A

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pirespuro2-CTLA4-mEos2

   Plasmid

   #98285

   Purpose

   mammalian expression of CTLA4-mEos2

   Depositor

   Mike Heilemann

   Article

   Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

   Insert

   CTLA4 (CTLA4 Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ220-Ultra

   Plasmid

   #176889

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

   Insert

   AsCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Plant

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME minprom_creERT2

   Plasmid

   #195966

   Purpose

   Gateway pME CreERT2 ORF

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mouse beta-globin minimal promoter creERT2
   

   Use

   Cre/Lox

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-pelB-GST-2

   Plasmid

   #228984

   Purpose

   For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-2
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-GST-1

   Plasmid

   #228983

   Purpose

   For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-1
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMVHA E2F2

    Plasmid

    #24226

    Purpose

    Mammalian expression of human E2F2 with HA tag

    Depositor

    Kristian Helin

    Article

    Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

    Insert

    E2F2 (E2F2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFloat.T7_v1

    Plasmid

    #133070

    Purpose

    Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 612 p3E-2A-mCherryCAAXpA

    Plasmid

    #195971

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-mCherry-CAAX (membrane)
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMuLE (L1-L4) 4X PRE

    Plasmid

    #206167

    Purpose

    pMuLE L1-L4 entry plasmid containing 4 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    4X PRE
    

    Use

    Gateway cloning entry clone

    Promoter

    2YBTATA

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221_HUWE1_isoform1

    Plasmid

    #190464

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. prDNA spacer-LacOx50-20xMS2-Hyg

    Plasmid

    #210219

    Purpose

    Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b_PstS_2

    Plasmid

    #78199

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C; A197C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-FAS-GCaMP6f-WPRE

    Plasmid

    #216277

    Purpose

    AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Promoter

    human synapsin 1 (hSyn)

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRetroX-Tight-MCS_PGK-GpNLuc

    Plasmid

    #70185

    Purpose

    Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pR-mKate2

    Plasmid

    #228610

    Purpose

    Mammalian expression of mKate2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mKate2
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRetroX-Tight-MCS_PGK-OgNLuc

    Plasmid

    #70186

    Purpose

    Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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